CORE--RECOMBINANT PROTEIN CORE

重组蛋白核心的组织是为了满足研究项目的需要，生产，纯化和表征蛋白质和抗体，这是不可用的其他地方。 此外，核心将产生以前从未产生过的新蛋白质和抗体。 四个基础科学和转化研究项目各自将需要生长因子、结合蛋白、受体、信号级联蛋白、激酶、转录因子、其突变形式及其抗体。 由于其专业性质，该核心将作为封闭核心管理。优先事项将由国际咨询委员会与核心主任协调确定。 重组蛋白和抗体的生产、纯化和表征的集中化将证明是高度成本有效的。 每个单位生产、纯化和鉴定自己的重组蛋白和抗体所需的重复工作、专业知识和设备将被消除，专业知识和技术将集中在核心。 核心主任的丰富经验将被带到承担每个研究项目的需求。 在同一种蛋白质或抗体需要被多个研究项目使用的情况下，对试剂的均匀性和质量的依赖将特别重要。 向研究项目11-14提供这些试剂将大大促进目标的推进，在某些情况下，将做出关键贡献，如果没有这些贡献，某些领域的研究将是不可能的。

The Recombinant Protein Core was organized to meet the needs of the Research Projects for production, purification and characterization of proteins and antibodies that are unavailable elsewhere. In addition, the Core will generate new proteins and antibodies never before produced. The four basic science and translational Research Projects each will require growth factors, binding proteins, receptors, signaling cascade proteins, kinases, transcription factors, mutant forms thereof, and antibodies thereto. This core will be administered as a closed core due to its specialized nature. Priorities will be set by the International Advisory Committee in coordination with the Core Director. The centralization of production, purification and characterization of recombinant proteins and antibodies will prove highly cost effective. The duplication of effort, expertise and equipment necessary for each unit to produce, purify and characterize their own recombinant proteins and antibodies will be eliminated and the expertise and technology concentrated in the Core. The extensive experience of the Core Director will be brought to bear on the needs of each Research Project. In cases where the same proteins or antibodies need to be used by more than one Research Project, reliance on the uniformity and quality of the reagents will be particularly important. Providing these reagents to Research Projects 11-14 will greatly facilitate the advancement of the aims and in some cases will make key contributions without which some areas of investigation would not be possible.