CORE--BIOCHEMISTRY AND BIOTECHNOLOGY

核心--生物化学与生物技术

• 批准号: 6652204
• 负责人: ANTHONY T YEUNG
• 金额: $ 19.62万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652204
• 关键词: aminoacid analyzer; biomedical facility; biomedical resource; high performance liquid chromatography; peptide chemical synthesis; protein sequence

Description: (Applicant's Description) The Biochemistry and Biotechnology Facility (BBF) makes available to Fox Chase investigators three state-of-the-art services that are connected to each other in research applications. DNA Technology provides uniformly high quality DNA, RNA, and modified oligonucleotides, the assembly of designer genes, and mutation detection by our patented process. Because of quality control, oligonucleotides from BBF have never been a variable in the users' experiments. DNA Technology leads to new genes and proteins that are further supported by the Biotechnology services of BBF. These include protein expression, fermentation, purification of enzymes and peptides, phage-displayed antibodies, and HPLC applications. A new addition is the BIAcore biosensor with which protein-ligand interactions are now investigated effectively. Through BBF, translational research projects gain instant access to equipment, manpower, and expertise in cutting edge technologies, and new investigators can quickly get up to speed. With institutional and CCSG developmental funds, the BBF has extended the above research with modern Mass Spectrometry. Services include high sensitivity protein analysis and identification, partial peptide sequencing, characterization of post-translational modifications, DNA analysis, drug chemistry, and enzyme characterization. In the cell, post-translational modifications of the proteins and changes in the level of key proteins provide important mechanisms whereby cellular processes are controlled. This important area of cancer biology is currently best addressed by combining 2D gel electrophoresis with mass spectrometry. BBF is using these techniques to assist many research programs at FCCC, and proposes to achieve even higher efficacy in the next CCSG cycle. BBF services support 52 peer-review funded principal investigators in all 13 Programs in all three Divisions. The staff of BBF is thoroughly cross-trained to assist each other. Because of the readiness of instruments, manpower, and experience, the efficacy of the BBF is impossible in the settings of individual laboratories. The BBF staff make frequent contact with the Facility users to determine how the Facility can best serve them. This energetic style of operation stimulates scientific interactions and the dissemination of ideas. Many projects have facilitated the obtaining of new peer-review support, new collaborations, publications, and patents.

描述：（申请人描述）生物化学和生物技术 设施（BBF）提供给福克斯大通调查人员三个 在研究中相互连接的最先进的服务 应用. DNA技术提供统一的高质量DNA，RNA， 修饰的寡核苷酸、设计基因的组装和突变 通过我们的专利工艺进行检测。由于质量控制， 来自BBF的寡核苷酸从未成为用户的变量。 实验DNA技术导致新的基因和蛋白质， 由BBF的生物技术服务支持。其中包括蛋白质 酶和肽的表达、发酵、纯化， 噬菌体展示抗体和HPLC应用。新增加的是 BIAcore生物传感器，用于研究蛋白质-配体相互作用 有效地通过BBF，转化研究项目获得即时访问 设备，人力和尖端技术的专业知识，以及新的 调查人员可以很快跟上进度。与机构和CCSG 发展基金，BBF已经扩展了上述研究与现代质量 光谱法服务包括高灵敏度蛋白质分析， 鉴定，部分肽测序，表征 翻译后修饰、DNA分析、药物化学和酶 特征化在细胞中， 蛋白质和关键蛋白质水平的变化提供了重要的机制 从而控制细胞过程。这个重要的癌症区域 生物学目前最好通过将2D凝胶电泳与 质谱分析法来BBF正在使用这些技术来协助许多研究 计划在FCCC，并建议在未来实现更高的效率， CCSG循环。 BBF服务支持52同行评审资助的主要研究者在所有13个 三个部门的方案。BBF的员工经过全面的交叉培训 互相帮助由于仪器、人力和 根据经验，BBF的疗效在以下情况下是不可能的： 个别实验室。BBF的工作人员经常与 设施使用者确定设施如何能最好地为他们服务。这 充满活力的工作方式促进科学的相互作用， 传播思想。许多项目促进了新项目的获得 同行评审支持、新的合作、出版物和专利。