CORE--BIOCHEMISTRY AND BIOTECHNOLOGY

描述：(申请人描述)生物化学和生物技术专业 设施(BBF)向福克斯大通调查人员提供三个 在研究中相互连接的最先进的服务 申请。DNA技术提供统一的高质量DNA、RNA和 修饰的寡核苷酸、设计基因的组装和突变 通过我们的专利工艺进行检测。由于质量控制， 来自BBF的寡核苷酸从来不是使用者的变量 实验。DNA技术导致新的基因和蛋白质进一步 由BBF的生物技术服务提供支持。其中包括蛋白质。 酶和多肽的表达、发酵、纯化， 噬菌体展示抗体及其高效液相色谱应用。新增加的是 目前研究蛋白质-配体相互作用的Biacore生物传感器 有效地。通过BBF，翻译研究项目获得即时访问 到尖端技术和新技术的设备、人力和专业知识 调查人员可以迅速掌握情况。与机构和CCSG合作 发展基金，BBF将上述研究与现代大众相结合 光谱分析。服务包括高灵敏度蛋白质分析和 鉴定、部分多肽测序、鉴定 翻译后修饰、DNA分析、药物化学和酶 人物刻画。在单元格中，对 蛋白质和关键蛋白质水平的变化提供了重要的机制 从而控制细胞的过程。癌症的这一重要领域 目前最好的解决生物学问题的方法是将2D凝胶电泳与 质谱学。BBF正在使用这些技术来帮助许多研究 计划，并提议在下一年实现更高的效率 CCSG循环。 BBF的服务在所有13个项目中支持52个同行评审资助的首席调查员 所有三个部门的计划。BBF的工作人员都经过了彻底的交叉培训 互相帮助。因为准备好了仪器、人力和 经验，BBF的功效是不可能的设置在 单独的实验室。BBF的工作人员经常与 设施用户确定设施如何才能最好地为他们提供服务。这 充满活力的运营方式刺激了科学互动和 思想的传播。许多项目为获得新的 同行评议支持、新的合作、出版物和专利。

Description: (Applicant's Description) The Biochemistry and Biotechnology Facility (BBF) makes available to Fox Chase investigators three state-of-the-art services that are connected to each other in research applications. DNA Technology provides uniformly high quality DNA, RNA, and modified oligonucleotides, the assembly of designer genes, and mutation detection by our patented process. Because of quality control, oligonucleotides from BBF have never been a variable in the users' experiments. DNA Technology leads to new genes and proteins that are further supported by the Biotechnology services of BBF. These include protein expression, fermentation, purification of enzymes and peptides, phage-displayed antibodies, and HPLC applications. A new addition is the BIAcore biosensor with which protein-ligand interactions are now investigated effectively. Through BBF, translational research projects gain instant access to equipment, manpower, and expertise in cutting edge technologies, and new investigators can quickly get up to speed. With institutional and CCSG developmental funds, the BBF has extended the above research with modern Mass Spectrometry. Services include high sensitivity protein analysis and identification, partial peptide sequencing, characterization of post-translational modifications, DNA analysis, drug chemistry, and enzyme characterization. In the cell, post-translational modifications of the proteins and changes in the level of key proteins provide important mechanisms whereby cellular processes are controlled. This important area of cancer biology is currently best addressed by combining 2D gel electrophoresis with mass spectrometry. BBF is using these techniques to assist many research programs at FCCC, and proposes to achieve even higher efficacy in the next CCSG cycle. BBF services support 52 peer-review funded principal investigators in all 13 Programs in all three Divisions. The staff of BBF is thoroughly cross-trained to assist each other. Because of the readiness of instruments, manpower, and experience, the efficacy of the BBF is impossible in the settings of individual laboratories. The BBF staff make frequent contact with the Facility users to determine how the Facility can best serve them. This energetic style of operation stimulates scientific interactions and the dissemination of ideas. Many projects have facilitated the obtaining of new peer-review support, new collaborations, publications, and patents.