CORE--OLIGONUCLEOTIDE SYNTHESIS AND FERMENTOR

核心--寡核苷酸合成及发酵罐

• 批准号: 6563721
• 负责人: DAN S RAY
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563721
• 关键词: DNA; biomedical automation; biomedical facility; microorganism culture; nucleic acid chemical synthesis; nucleic acid probes; nucleic acid sequence; oligonucleotides; synthetic nucleic acid

Description: The Oligonucleotide Synthesis and Fermentor Shared Resource consists of two distinct components: an Oligonucleotide Synthesis Facility and a Fermentor Facility. The facility has been operational for over 10 years. The original facility, which had a single synthesizer, has now been expanded to include three different machines, capable of synthesizing a total of eight oligonucleotides at a time. Most of the support of this facility is derived from recharges to research grants. The current recharge rates are competitive with those of commercial companies for small scale synthesis of short oligonucleotides, and significantly cheaper for large scale synthesis of longer oligonucleotides and specially modified bases. In general, the application stated that it was possible to receive oligonucleotides on the same day of order, and in general the turn around time was one to two days for custom syntheses. Of note in the usage of the facility is that after a decline in usage in 1993-94 and 1994-95, usage increase in 1995-96. The facility will gel purify oligonucleotides at no additional charge upon request. Seventeen different investigators requested synthesis of oligonucleotides with modified bases. The fermentation facility maintains and operates two fermentors, one of 12 liter pilot scale and a second of 170 liter large scale capacity. The purpose of the facility is to offer large quantities of microbial cells for enzyme purifications and recombinant proteins for physical studies. The chargeback to investigators using the facility was significantly less than that cited for commercial sources. Usage of the facility included 26 charges to Center members, and 43 to non-members.

描述：寡核苷酸合成和发酵罐共享资源 由两个不同的部分组成：寡核苷酸合成设施和 a发酵罐设施。该设施已经运行了10多年。 最初只有一个合成器的设施现在已经扩大了 包括三个不同的机器，能够合成总共八个 寡核苷酸一次。该设施的大部分支持来自 从充值到研究赠款目前的充值费率具有竞争力 与商业公司的小规模合成短 寡核苷酸，并且对于大规模合成来说显着更便宜 更长的寡核苷酸和特别修饰的碱基。总体上 该申请指出，可以在细胞上接收寡核苷酸。 订单当天，一般来说，周转时间为一到两天， 定制合成。在使用该设施时值得注意的是， 一九九三至九四年度及一九九四至九五年度使用量下降，一九九五至九六年度则增加。的 设施将凝胶纯化寡核苷酸，不收取额外费用， 请求过程中17名不同的研究者要求综合 具有修饰的碱基的寡核苷酸。 发酵设施维护和操作两个发酵罐，12个发酵罐之一， 第二个是170升的中试规模，第二个是170升的大规模容量。目的 该设施的主要目的是为酶提供大量的微生物细胞 用于物理研究的纯化和重组蛋白。使用计费 对使用该设施的调查人员来说， 商业来源。设施的使用包括向中心收取的26项费用 43人，非党员。