Anti-Inflammatory Properties of Cannabis Constituents

大麻成分的抗炎特性

• 批准号: 6724672
• 负责人: ROBERT B ZURIER
• 金额: $ 19.88万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6724672
• 关键词: Cannabis; acute disease /disorder; antiinflammatory agents; cannabinoids; chronic disease /disorder; clinical research; cytokine; drug design /synthesis /production; drug discovery /isolation; drug screening /evaluation; eicosanoids; enzyme linked immunosorbent assay; high performance liquid chromatography; human subject; inflammation; laboratory rat; mass spectrometry; oral administration; plant extracts; polyarthritis; thin layer chromatography

DESCRIPTION (provided by applicant): The overall long term objective of this proposal is to determine whether individual nonpsychoactive constituents of Cannabis sativa (marijuana), alone or in combination, will provide safe, effective treatment for diseases characterized by acute and/or chronic inflammation. The medicinal use of marijuana has been limited by its psychoactivity and by induction of tolerance. Existence of nonpsychoactive marijuana constituents and development of synthetic cannabinoids encourage further research to identify nonpsychoactive plant components with antiinflammatory properties. A complete marijuana extract (CME) and pure nonpsychoactive cannabinoid and noncannabinoid Cannabis constituents will be tested in human whole blood models for their ability to influence production in vitro of inflammatory eicosanoids and cytokines. The antiinflammatory effects of oral administration of the CME and the pure nonpsychoactive Cannabis constituents will also be tested in a model of acute inflammation (induced by IL-1Beta and TNFalpha in a subcutaneous air pouch in rats), and a model of chronic inflammation (adjuvant induced polyarthritis in rats). Controls for experiments in vitro and studies in animals will include vehicle, dexamethasone, indomethacin, and a selective inhibitor of cyclooxygenase 2. A noncannabinoid, nonpsychoactive marijuana constituent termed cannflavin will be isolated using high-pressure liquid chromatography and tested in the in vivo models and in vitro systems. Successful completion of the proposed experiments should identify ways of harnessing important antiinflammatory properties of marijuana without the limiting adverse effects.

描述（由申请人提供）：本提案的总体长期目标是确定大麻（大麻）的单个非精神活性成分单独或组合是否将为以急性和/或慢性炎症为特征的疾病提供安全有效的治疗。大麻的药用因其精神活性和诱导耐受性而受到限制。大麻非精神活性成分的存在和合成大麻素的发展鼓励进一步研究鉴定具有抗炎特性的非精神活性植物成分。将在人体全血模型中测试一种完整的大麻提取物（CME）和纯非精神活性大麻素和非大麻素大麻成分对体外炎性类二十烷酸和细胞因子产生影响的能力。