Polytene chromosomes of Culex pipiens mosquito complex

描述(由申请人提供)：库蚊复合体的成员分布在世界各地，在不同地区是圣路易斯脑炎、西尼罗河和裂谷热病毒以及班克罗夫氏伍氏杆菌的媒介。该复合体的两个成员，即CX。Popiens和Cx.它们的行为并不一致，这与它们对疾病的媒介能力的差异性有关。这些亚种在取血亲和力(哺乳动物和鸟类)、自体繁殖程度、休息行为(嗜内性和外嗜性)、对病毒的媒介能力和交配行为等方面存在差异。因此，有必要更清楚地界定CX成员的人口结构。罗汉果复合体。多线染色体重排可以作为一种工具，更清楚地描述和确定亚种内和亚种之间的基因流动。这项建议的总体目标是描述Cx成员的唾液腺多线染色体的特征。Popens Complex.具体目标是：1)制作Cx唾液腺染色体的照片图。Popiens、Cx.P.ququiefasciatus和Cx.P.molestus和2)确定Cx.Popiens、Cx.P.ququiefasciatus和Cx.P。猥亵。

DESCRIPTION (provided by the applicant): Members of the Culex pipiens mosquito complex have a worldwide distribution and in various regions are vectors of St. Louis encephalitis, West Nile and Rift Valley fever viruses, and Wuchereria bancrofti. Two members of the complex, namely, Cx. p. pipiens and Cx. p. quinquefasciatus, are not uniform in their behavior, which is associated with variability in their vectorial capacities for diseases. These subspecies vary, in blood feeding avidity (mammal vs birds), degree of autogeny, resting behavior (endophilic vs. exophilic), vector competence for viruses and mating behavior. There is hence a need to more clearly define the population structure of members of the Cx. pipiens complex. Polytene chromosome rearrangements can serve as a tool to more clearly characterize and determine gene flow both within and between the subspecies. The overall objective of this proposal is to characterize the salivary gland polytene chromosomes of members of the Cx. p. pipens complex. The specific aims are: 1) Produce photomaps of salivary gland chromosomes of Cx. p. pipiens, Cx. p. quinquefasciatus and Cx. p. molestus and 2) Determine chromosomal homologies between Cx. p. pipiens, Cx. p. quinquefasciatus and Cx. p. molestus.