权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Structural and functional interactions of mitochondrial haemoglobin and ATP synthase in ageing

衰老过程中线粒体血红蛋白和 ATP 合酶的结构和功能相互作用

基本信息

批准号：
2275296
负责人：
金额：
--
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2275296
关键词：
Structural functional interactions mitochondrial haemoglobin

项目摘要

We recently described the presence of haemoglobin in the mitochondrial organelle (Shephard et al.,2014 and 2016). This finding has major implications in our understanding of energy production in the cell. The Antarctic 'white' ice fish (from the sub-order Notothenioid) have white blood which lacks the protein haemoglobin. Through our collaborators we will be able to analyse tissues from these and closely related red fish that do have haemoglobin. The student will examine the mitochondrial haemoglobin interactome proteins, their expression/distribution and compare mitochondrial enzyme complex function in both the red and white fish. The student will also examine the sub-mitochondrial localisation of these proteins. In parallel, the student will examine the effect of knock-down of the glob in proteins in C.elegans. Globin knockdowns will be examined for changes in mitochondrial form and function using our mitoGFP strain and high resolution respirometry.The student will carry out molecular/biochemical work as part of the Chakrabarti group. The second part is supervised by Dave Scott in Biosciences/RCaH and will examine the potential interactions of mitochondrial haemoglobin using fluorescent super resolution microscopy techniques. Fifty percent will be based at the Diamond Light Source and supervised by Peijun Zhang. State of the art cryo-electron microscopy techniques will be used to elucidate the mitochondrial phenotype of the mutant species.

我们最近描述了线粒体细胞器中血红蛋白的存在（Shephard等人，2014年和2016年）。这一发现对我们理解细胞中的能量产生具有重要意义。南极的“白色”冰鱼（来自南极鱼亚目）有白色的血液，缺乏蛋白质血红蛋白。通过我们的合作者，我们将能够分析这些组织和密切相关的红鱼，确实有血红蛋白。学生将检查线粒体血红蛋白相互作用蛋白质，它们的表达/分布，并比较线粒体酶复合体在红色和白色鱼中的功能。学生还将研究这些蛋白质的亚线粒体定位。与此同时，学生将研究敲低线虫蛋白质中的球的效果。将使用我们的mitoGFP菌株和高分辨率呼吸测定法检查珠蛋白敲低对线粒体形式和功能的变化。学生将作为Chakrabarti小组的一部分进行分子/生物化学工作。第二部分由生物科学/RCaH的Dave Scott监督，并将使用荧光超分辨率显微镜技术研究线粒体血红蛋白的潜在相互作用。其中50%将在钻石光源工作，由张培军监督。将使用最先进的冷冻电子显微镜技术阐明突变种属的线粒体表型。