Quantum Cascade Laser s-SNOM for intracellular imaging

用于细胞内成像的量子级联激光 s-SNOM

• 批准号: 2277215
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2277215
• 关键词: Quantum; Cascade; Laser; SNOM; intracellular

Very recently we gave found out how to use a Solid- State near field imaging technique, so-called s-SNOM to look inside cells for the first time. It beats the diffraction limits of ordinary microscopy by a factor of ~3000, and the spatial resolution it gives (~3nm) already rivals the very best of electron microscopy at a fraction of the time effort and cost. We believe it has the potential to transform the biomedical sciences.We have shown how it can image the organelles inside a cell optically for the first time first time ever. Also , because it works at mid-IR wavelengths where chemical bonds have characteristic vibrational absorption bands that give them spectral "fingerprints" , the technique can be used to provide nanoscal chemnical maps that, e.g. reveal where drugs bind inside the cell.The initial aims of the projet will be to trail and develop the technology with Imperial cinicians, with a focus on themes, (1) the pathology pf Breast Cancer, (2) Drug resistance in multiple myeloma, and (3) the nanoscale causes of osteporosis. However, the potential applications are limitless and we will likely establish more collaborations as the programme progresses.Also Recent developments in the field of quantum imaging have demonstrated new methods of imaging objects by detecting photons that have not actually interacted with it. In collaboration with colleagues in the optics groups and the QUANTIC2 Quantum Imaging Hub, we plan to investigate extending the wavelength of this technique into the mid-IR.There will also be collaboration with the team developing the diffraction-limited "Digistain" imager for Cancer diagnosis.

最近，我们发现了如何使用固态近场成像技术，即所谓的s-SNOM来首次观察细胞内部。它超越了普通显微镜的衍射极限约3000倍，空间分辨率（约3 nm）已经可以与最好的电子显微镜相媲美，而时间和成本却只有电子显微镜的一小部分。我们相信它有潜力改变生物医学科学。我们已经展示了它是如何有史以来第一次用光学方法成像细胞内的细胞器的。此外，由于它工作在中红外波长，化学键具有特征振动吸收带，使它们具有光谱“指纹”，该技术可用于提供纳米尺度的化学图谱，例如揭示药物在细胞内的结合位置。该项目的最初目标是与帝国电影工作者一起跟踪和开发该技术，重点关注主题，（1）乳腺癌的病理学，（2）多发性骨髓瘤的耐药性，以及（3）骨质疏松症的纳米级原因。然而，潜在的应用是无限的，随着计划的进展，我们可能会建立更多的合作。此外，量子成像领域的最新发展已经展示了通过检测实际上没有与物体相互作用的光子来成像物体的新方法。在与光学组和QUANTIC 2量子成像中心的同事的合作中，我们计划研究将这种技术的波长扩展到中红外。2我们还将与开发用于癌症诊断的衍射限制“Digistain”成像仪的团队合作。