Advanced dev of the constant depth film fermenter towards optimised biofilm modelling and development of functional insights of biofilm communities

恒定深度膜发酵罐的高级开发，以优化生物膜建模并开发生物膜群落的功能见解

• 批准号: 2277258
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2277258
• 关键词: Advanced; dev; constant; depth; film

The oral cavity is highly populated with bacterial species, and around 700 oral bacterial species have been identified. Dental plaque is related to the aetiology of two of the most prevalent disease: caries and periodontal diseases. According to the ecological plaque hypothesis not only one or few bacterial species, but the disturbance of the symbiotic interactions between the host and the resident oral microbiota, as well as the microbial homeostasis are responsible for the occurrence of oral infections. Therefore, constant mechanical removal and improved oral hygiene are essential to prevent the occurrence of oral infections. The School of Dentistry at the University of Liverpool has unique expertise in the use of the constant-depth film fermenter (CDFF) model which combines the complexity of a physiologically relevant multispecies biofilm with a simulation of the abrasion and flow dynamics present in the mouth. The CDFF therefore is a specialised platform producing supragingival plaque with a composition that is representative of those occurring in vivo when it is inoculated with a microcosm culture from donated saliva. We plan to combine the world class expertise of both the School of Dentistry and the Centre of Genomic Research (CGR/MIF-OMICS) for genomic analysis and sequencing. The centre has experience in characterising the human microbiome in a variety of contexts including the mouth, one of the most diverse microbiomes in the body, and advanced insights in its diversity and association to health and disease. The aim of this project is to open a new area of research into the characterisation and development of more representative oral microbiome models. In the process we envisage the prospective student to get exposure and training in a rich diversity of techniques by utilising the state of art facilities in both departments.The proposed PhD studentship squarely fits within the World Class Underpinning Bioscience remit of BBSRC, specifically within Fundamental Bioscience and exploit new ways of working.

口腔中有大量的细菌，已经鉴定出约700种口腔细菌。牙菌斑与龋病和牙周病这两种最常见的疾病的病因有关。根据生态菌斑假说，不仅一种或几种细菌物种，而且宿主和常驻口腔微生物群之间的共生相互作用的干扰，以及微生物体内平衡是导致口腔感染发生的原因。因此，持续的机械清除和改善口腔卫生对于预防口腔感染的发生至关重要。利物浦大学牙科学院在使用恒定深度膜发酵罐（CDFF）模型方面拥有独特的专业知识，该模型将生理相关的多物种生物膜的复杂性与口腔中存在的磨损和流动动力学的模拟相结合。因此，CDFF是一种产生龈上菌斑的专门平台，其组成代表了当用来自捐献唾液的微宇宙培养物接种时体内发生的组成。我们计划将牙科学院和基因组研究中心（CGR/MIF-OMICS）的世界级专业知识联合收割机用于基因组分析和测序。该中心在多种环境下表征人类微生物组方面拥有丰富的经验，包括口腔（体内最多样化的微生物组之一），并对其多样性及其与健康和疾病的关联有着先进的见解。该项目的目的是开辟一个新的研究领域，以表征和开发更具代表性的口腔微生物组模型。在这个过程中，我们设想未来的学生通过利用两个部门的最先进的设施，获得丰富多样的技术的接触和培训。拟议的博士生名额完全符合BBSRC的世界级基础生物科学职权范围，特别是在基础生物科学和探索新的工作方式。