Processing of Pre-Messenger RNA

前信使 RNA 的加工

• 批准号: 6780836
• 负责人: SUSAN M BERGET
• 金额: $ 50.14万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6780836
• 关键词: RNA binding protein; RNA splicing; cell line; gene induction /repression; genetic enhancer element; genetic regulation; genetic regulatory element; helicase; introns; laboratory rat; nucleic acid biosynthesis; nucleic acid sequence; precursor mRNA; protein binding; protein protein interaction; protein purification; spliceosomes; transcription factor

DESCRIPTION (provided by applicant): Alternative pre-mRNA splicing contributes significantly to genetic diversity in vertebrates. This proposal is aimed at understanding the mechanisms underlying the regulation of alternative pre-mRNA splicing. Alternatively spliced regions of a pre-mRNA are characterized by the presence of splicing enhancer or silencer sequence elements recognized by splicing factors. Our work concentrates on four systems of pre-mRNA splicing that involve different types of regulatory elements and splicing factors. Included in the RNA regulatory sequence elements to be studied are: 1) C/A-rich exon enhancers that stimulate exon inclusion, G/A-rich exon enhancers that stimulate exon inclusion and regulate 5' splice site usage, G-rich intron enhancers that regulate 5' splice site usage, and C-rich intron elements that stimulate splicing and spliceosome assembly. Experiments are described to: 1) continue characterization of the factors that recognize these RNA elements, 2) identify and characterize factors that recognize element-binding factors to regulate splicing, 3) determine the role of RNA helicases in alternative splicing and 4) investigate the hypothesis that alternative splicing and transcriptional elongation are linked.

描述（由申请人提供）：可选的前mrna剪接对脊椎动物的遗传多样性有重要贡献。这一建议的目的是了解潜在的机制调控选择性前mrna剪接。前mrna的选择性剪接区域的特征在于剪接因子识别的剪接增强子或沉默子序列元件的存在。我们的工作集中在四个前mrna剪接系统，涉及不同类型的调控元件和剪接因子。待研究的RNA调控序列元件包括：1)刺激外显子包含的富含C/ a的外显子增强子，刺激外显子包含并调节5‘剪接位点使用的富含G/ a的外显子增强子，调节5’剪接位点使用的富含G的内含子增强子，以及刺激剪接和剪接体组装的富含C的内含子元件。实验描述为：1)继续表征识别这些RNA元件的因子；2)识别和表征识别元件结合因子以调节剪接的因子；3)确定RNA解旋酶在选择性剪接中的作用；4)研究选择性剪接与转录延伸相关的假设。