BMP signaling and cell fate in the embryonic endoderm

胚胎内胚层中的 BMP 信号传导和细胞命运

• 批准号: 6626138
• 负责人: AIDAN J PETERSON
• 金额: $ 4.64万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-12-15 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626138
• 关键词: biological signal transduction; bone morphogenetic proteins; cell differentiation; endoderm; extracellular; fibroblast growth factor; gene expression; genetic markers; genetic promoter element; genetic regulation; genetic regulatory element; genetically modified animals; histogenesis; laboratory mouse; liver cells; pancreas; polymerase chain reaction; postdoctoral investigator; thyroid gland; transcription factor

DESCRIPTION (provided by applicant) The goal of this proposal is to learn how extracellular signaling molecules direct the formation of specialized cell types during embryonic development. The role of bone morphogenetic proteins (BMPs) in early liver development will be studied using a mouse tissue explant culture assay to determine how uncommitted cells in the endoderm are directed to become liver cells. The role of BMP in the specification of other endoderm organ cell types, including pancreas and thyroid, will be studied to determine if this signaling molecule is dedicated to liver development or plays a general role in endoderm organ formation. Signal transduction components of the BMP pathway and the fibroblast growth factors (FGFs) pathway, another signal that promotes liver development, will be monitored to determine if one of these signals permits a target cell to process the other signal. The role of transcription factors that are activated by BMPs will be examined to learn how they cooperate with endoderm factors to carry out BMP-dependent gene regulation. This project is designed to provide a multifaceted view of how a precursor endoderm cell processes an extracellular BMP signal and is committed to a liver fate. Knowledge of natural cell differentiation will promote the goal of manipulating cell differentiation for medical purposes.

描述（由申请人提供） 该建议的目的是了解细胞外信号分子如何 指导在胚胎发育过程中形成专门的细胞类型。 骨形态发生蛋白（BMP）在早期肝发育中的作用将 使用小鼠组织外植体培养测定法进行研究，以确定如何 内胚层中未承诺的细胞被指示成为肝细胞。角色 BMP在其他内毒剂细胞类型的规范中，包括 将研究胰腺和甲状腺，以确定该信号分子是否 致力于肝脏发育或在内胚中发挥一般作用 形成。 BMP途径和成纤维细胞的信号转导组件 生长因子（FGFS）途径，这是促进肝发育的另一个信号， 将监视以确定这些信号之一是否允许目标单元 处理另一个信号。被激活的转录因子的作用 将检查BMP，以了解他们如何与内胚层因素合作 执行依赖BMP的基因调节。该项目旨在提供 前体内胚层细胞如何处理细胞外的多方面视图 BMP信号并致力于肝脏命运。自然细胞的知识 分化将促进操纵细胞分化的目标 医疗目的。