Development of cell-based drug discovery assays using high-throughput MALDI TOF mass spectrometry

使用高通量 MALDI TOF 质谱法开发基于细胞的药物发现分析

• 批准号: 2281884
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2281884
• 关键词: Development; cell; based; drug; discovery

"Mass spectrometry (MS) is an emerging technology for identifying and characterizing drugs that modulate biological targets, offering a label-free, direct detection method. This technology enables the application of more physiologically relevant assays and reduces time and costs compared to current classical approaches increasing the efficiency of the drug discovery process. In the past, the throughput of MS-based assay technologies was limited, but recent developments in the field of MALDI-TOF-MS devices and spotting technologies substantially increased the ability for miniaturization and speed of such approaches. However, the application of MALDI is based on a matrix-compatible sample preparation step and is limited to a certain space of analytes. This requires the identification of MALDI-compatible, physiological relevant assay conditions, as well as development of fast and reproducible liquid handling procedures. The PhD work will focus on the evaluation to extend the application of MALDI-TOF-MS to cell-based assays in high-throughput screening and investigate chemical modifications to increase "

“质谱（MS）是一种新兴的技术，用于识别和表征调节生物靶标的药物，提供无标记的直接检测方法。与目前的经典方法相比，该技术能够应用更生理相关的测定，并减少时间和成本，提高药物发现过程的效率。在过去，基于MS的测定技术的通量是有限的，但MALDI-TOF-MS装置和点样技术领域的最新发展大大提高了这种方法的小型化和速度的能力。然而，MALDI的应用是基于基质相容的样品制备步骤，并且限于一定空间的分析物。这需要识别MALDI兼容的生理相关测定条件，以及开发快速和可重复的液体处理程序。博士工作将集中在评估，以扩大应用MALDI-TOF-MS的细胞为基础的分析在高通量筛选和调查化学修饰，以增加“