Development of Debaryomyces hansenii as a robust host for production of high value fatty acid-based biochemicals.

石油化学衍生的生物化学品在全球范围内使用，然而，世界范围内正在推动用可持续的生物对应物取代它们。自然界提供了许多工具来合成种类繁多的生物化学物质，但是在工业上收获这些化合物时出现了诸如产量低、致病性和培养生物体困难等问题。工业生物技术界经常面临的挑战是如何操纵细胞合成机器以产生可最佳利用的产品。在这个合成生物学项目中，你将成为一个跨学科团队的一员，该团队对使用非致病性酵母Debaryomyceshansenii作为生产宿主生产一类生物乳化剂感兴趣。你将讨论耐海水胁迫细胞如何适应可能使其膜乳化的化合物的生产。您将引入产生乳化剂的基因到D. hansenii中，然后使用各种技术解决这个问题，包括转录组学分析，脂质组学和代谢途径分析。然后，您的发现将用于重新设计细胞生物合成途径，以改善生物乳化剂的生产。这个项目是基于工业生物技术的原则，但创造了与分子生物学家，生物信息学家，化学家和生理学家一起工作的机会。

Petro-chemically derived biochemicals are used globally, however,there is a worldwide drive to replace them with sustainable,biological counterparts. Nature provides many tools to synthesise ahuge variety of biochemicals but there are problems which arisewhen industrially harvesting these compounds such as low yields,pathogenicity and difficulty in culturing the organisms. Thechallenge frequently faced by the industrial biotechnologycommunity is how to manipulate cellular synthesis machinery inorder to yield products which can be used optimally.In this synthetic biology project you will be part of aninterdisciplinary team that is interested in the production of a classof bio-emulsifiers using the non-pathogenic yeast Debaryomyceshansenii as production host. You will address how the marinestress resistant cells adapt to the production of compounds thatpotentially emulsify their membranes. You will introduce the genesto produce the emulsifiers into D. hansenii and then address thequestion using a variety of techniques including transcriptomeanalysis, lipidomics and metabolic pathway analyses. Your findingswill then be used to re-engineer the cellular biosynthesis pathwayto improve bio-emulsifier production. This project is based aroundprinciples of industrial biotechnology but creates opportunities towork together with molecular biologists, bioinformaticians, chemistsand physiologists.