Biogenic Amine Transporters and G-protein Signaling

生物胺转运蛋白和 G 蛋白信号传导

• 批准号: 6645858
• 负责人: KERRY O Grimberg
• 金额: $ 3.37万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6645858
• 关键词: G protein; G protein coupled receptor kinase; analog; autoradiography; biological signal transduction; dopamine; laboratory rat; neuropharmacology; neurotransmitter receptor; neurotransmitter transport; norepinephrine; predoctoral investigator; receptor binding; receptor coupling; serotonin

DESCRIPTION (provided by applicant): The illicit use of cocaine continues to be a significant social problem. Although some of the chronic effects of psychostimulants have been well characterized, the neurochemical adaptations that occur in brain as a result of chronic drug use have not yet been elucidated. These experiments will characterize the effects of biogenic amine transporter blockade on receptors and receptor coupling to G-proteins in brain, which will provide further insight into biogenic amine systems. Specifically, the objectives of this project are to test the following hypotheses: 1) Long-term blockade of biogenic monoamine transporters desensitizes biogenic amine receptor/G-protein coupling in specific brain regions, 2) chronic treatment with long acting biogenic amine transport blockers produces a long-term blockade of biogenic amine transporters, 3) receptor/G-protein desensitization after chronic WF-23 treatment may result in downregulation of biogenic amine receptors, and 4) chronic treatment with WF-23 or cocaine may produce changes in receptor reserve for D2 receptors. Investigation of the neuronal mechanisms underlying chronic transporter blockade will provide insight into drug abuse and potentially provide the information necessary to create better treatment programs for psychostimulant abuse.

描述（由申请人提供）：