Cell Removal during Programmed Cell Death

描述（由申请人提供）：自噬是动物发育过程中发生的程序性细胞死亡的一种主要形式。自噬的机制还没有得到很好的研究，尽管许多证据将自噬与人类疾病如帕金森氏症和癌症联系起来。类固醇激素20-羟基蜕皮激素在果蝇变态期间触发唾液腺细胞死亡。唾液腺细胞死亡在蛹形成后12小时开始，并在16小时完成。垂死的唾液腺细胞类似于经历自噬性细胞死亡的细胞。在此期间，这些细胞在形态上发生了巨大的变化。这项建议的目的是分析细胞的变化，发生在唾液腺组织溶解，以及确定新的基因，可能会在重塑过程中唾液腺自噬细胞死亡。

DESCRIPTION (provided by applicant): Autophagy is a prominent form of programmed cell death that occurs during animal development. The mechanism of autophagy has not been well studied, even though many lines of evidence link autophagy to human diseases such as Parkinson's and cancer. The steroid hormone 20-hydroxyecdysone triggers salivary gland cell death during Drosophila metamorphosis. Salivary gland cell death begins 12 hours after puparium formation and is complete by 16 hours. The cells of dying salivary glands resemble cells undergoing autophagic cell death. These cells undergo dramatic changes in morphology during this time. The aim of this proposal is to analyze cell changes that occur during salivary gland histolysis as well as to identify new genes that may act in the process of remodeling during salivary gland autophagic cell death.