Development of a whole cell biocatalyst designed to perform hydrogen-dependent carbon dioxide reduction under pressure.

该项目将专注于开发和表征细菌碳捕获系统，该系统利用酶复合物甲酸氢裂解酶（FHL）从气态CO2和H2中产生甲酸盐，同时细胞在加压反应器中生长。开发的系统还需要能够在一氧化碳存在的情况下运行。本课题的主要目的是：研究黑腐果胶杆菌（Pectobacterium atrospeticum）的甲酸氢解酶（FHL-2）复合物在大肠杆菌中的活性，验证FHL-2在大肠杆菌中具有完全功能的假说。设计、组装和表达一氧化碳脱氢酶（CODH）复合物，并在大肠杆菌中表达，该复合物具有氢依赖性CO2还原酶（HDCR）的功能。本节旨在检验来自泛菌属细菌的CODH可以在大肠杆菌中成功表达的假设。构建了一种新型的氢依赖型二氧化碳还原细胞生物催化剂。本节旨在测试以下假设：将活性CODH添加到表达FHL-2的细菌系统中将产生能够在一氧化碳存在下还原氢依赖性二氧化碳的生物催化剂。

The project will focus on developing and characterising a bacterial carbon capture system that utilises the enzyme complex formate hydrogenlyase (FHL) to generate formate from gaseous CO2 and H2 whilst cells grow in a pressurised reactor. The developed system will also need be capable of functioning in the presence of carbon monoxide. The key aims of this project are:To characterise the activity of the formate hydrogenlyase (FHL-2) enzyme complex from the bacterium Pectobacterium atrospeticum in Escherichia coli.To test the hypothesis that FHL-2 is fully functional within E. coli and can operate as a hydrogen-dependent CO2 reductase (HDCR) to generate formate form gaseous hydrogen and carbon dioxide.To design, assemble and characterise a synthetic Carbon Monoxide Dehydrogenase (CODH) enzyme complex in E. coli.This section aims to test the hypothesis that the CODH from a Pantoea species of bacteria can be successfully expressed in E. coli and can generate carbon dioxide from carbon monoxide.To assemble and characterise a novel hydrogen-dependent carbon dioxide reducing cell-based biocatalyst. This section aims to test the hypothesis that the addition of an active CODH into a bacterial system expressing FHL-2 will produce a biocatalyst capable of hydrogen-dependent carbon dioxide reduction in the presence of carbon monoxide.