Genotyping of the parasite Trichomonas vaginalis

寄生虫阴道毛滴虫的基因分型

• 批准号: 6738132
• 负责人: PETER UPCROFT
• 金额: $ 7.63万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6738132
• 关键词: Trichomonas vaginalis; chromosomes; drug resistance; fluorescent in situ hybridization; gene expression; genetic markers; genetic polymorphism; genetic strain; genotype; in situ hybridization; karyotype; linkage mapping; microorganism classification; molecular pathology; parasitic diseases; pulsed field gel electrophoresis; restriction endonucleases; sexually transmitted diseases; virulence

DESCRIPTION (provided by the applicant): The overall goal of this project is to establish the first successful comprehensive genotyping system to clearly distinguish at the genetic level individual, drug resistant and virulent isolates of the flagellated protozoan parasite Trichomonas vaginalis for future epidemiological and biochemical studies of pathogenesis, virulence and drug resistance. The genotyping system will also be applied to linkage studies, mapping the genome, and determining variability among genomes including the detection of drug resistant, pathogenic and virulent strains. Trichomoniasis is the most common, sexually transmitted infection. Symptoms include virginities and acute inflammatory disease of the genital mucosa, and infections have been associated with pre-term delivery, low birth weight and increased infant mortality, as well as predisposing to HIV/AIDS and cervical cancer. Trichomoniasis has the highest prevalence and incidence of any STI, and its eradication may well be the single most cost-effective step in HIV incidence reduction. This proposal utilises pulsed field gel electrophoresis of very large chromosomal DNA segments cleaved by restriction endonucleases of the Trichomonas genome, combined with gene hybridization, to generate a genotyping system, which distinguishes individual isolates. Such an analysis has not previously been achieved due to extensive endogenous nuclease activity. The genotyping system will be coupled to in situ hybridization of whole chromosomes and electrophoretic karyotyping for gene linkage studies, mapping genome organization and structure, determining genome variability among isolates, and defining markers for virulence and drug resistance.

描述(由申请人提供)：这个项目的总体目标是建立第一个成功的全面基因分型系统，在遗传水平上清楚地区分鞭毛虫原生动物寄生虫阴道毛滴虫的个体、抗药性和毒力菌株，用于未来发病机制、毒力和抗药性的流行病学和生化研究。该基因分型系统还将应用于连锁研究、绘制基因组图和确定基因组之间的变异性，包括检测耐药、致病和毒力菌株。滴虫病是最常见的性传播感染。症状包括童贞和生殖器黏膜的急性炎症性疾病，感染与早产、低出生体重和婴儿死亡率增加以及艾滋病毒/艾滋病和宫颈癌的易感性有关。滴虫病是所有性传播感染中患病率和发病率最高的，根除滴虫病很可能是减少艾滋病毒发病率的最具成本效益的单一步骤。这项建议利用脉冲场凝胶电泳法对被毛滴虫基因组的限制性内切酶切割的非常大的染色体DNA片段进行分析，结合基因杂交，产生一个基因分型系统，以区分不同的分离株。由于广泛的内源核酸酶活性，这种分析以前没有实现过。该基因分型系统将与全染色体原位杂交和电泳法核型相结合，用于基因连锁研究，绘制基因组组织和结构图，确定菌株之间的基因组变异，并确定毒力和耐药性的标记。