Cadherins in the Developing Zebrafish Inner Ear

发育中的斑马鱼内耳中的钙粘蛋白

• 批准号: 6822253
• 负责人: JAMES A MARRS
• 金额: $ 29.68万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6822253
• 关键词: acoustic nerve; alternatives to animals in research; cadherins; developmental genetics; developmental neurobiology; embryogenesis; ganglions; gene expression; gene targeting; immunocytochemistry; in situ hybridization; labyrinth; mesenchyme; neural crest; neurogenesis; oligonucleotides; otocyst /otolith; semicircular duct; zebrafish

DESCRIPTION (provided by applicant): We propose to examine inner ear development using zebra fish, Danio rerio, as a model system. Zebra fish has emerged as a powerful experimental organism because of the ease of use in developmental biology and genetic studies. The zebra fish inner ear is essentially the same as that in other vertebrates: a closed epithelial structure with three communicating chambers and three semicircular canals. However, no cochlea or cochlear duct is found in the fish ear. Sensory patches are found within the chambers and canals, composed of hair cells and supporting cells. Hair cells synapse with statoacoustic ganglion (SAG) cells that relay the signals to the brain. Otoliths in the matrix above the sensory patches allow teleost fish to detect mechanical stimulation, including linear and rotational acceleration, gravity and sound. Cadherin cell adhesion molecules control a variety of developmental processes, including processes like cell sorting, differentiation and neurite formation in the nervous system. We propose to determine the role of cadherin cell adhesion molecules during the development of the inner ear and SAG. Three specific aims will be employed; (1) determine expression pattems of cadherins in the inner ear of developing zebra fish; (2) determine the function of cadherins during inner ear development; and (3) determine the effects of Pax-2 gene function on cadherin expression during otic placode induction, inner ear development and statoacoustic ganglion (SAG) formation.

描述（由申请人提供）：我们建议使用斑马鱼 Danio rerio 作为模型系统来检查内耳发育。由于在发育生物学和遗传学研究中易于使用，斑马鱼已成为一种强大的实验生物。斑马鱼的内耳与其他脊椎动物的内耳基本相同：具有三个连通室和三个半规管的封闭上皮结构。然而，鱼耳中没有发现耳蜗或耳蜗管。感觉斑块存在于腔室和耳道内，由毛细胞和支持细胞组成。毛细胞与静声神经节 (SAG) 细胞形成突触，将信号传递给大脑。感觉斑块上方基质中的耳石使硬骨鱼能够检测机械刺激，包括线性和旋转加速度、重力和声音。钙粘蛋白细胞粘附分子控制多种发育过程，包括神经系统中的细胞分选、分化和神经突形成等过程。我们建议确定钙粘蛋白细胞粘附分子在内耳和 SAG 发育过程中的作用。将采用三个具体目标； (1)确定发育中斑马鱼内耳中钙粘蛋白的表达模式； (2)确定钙粘蛋白在内耳发育过程中的功能； (3)确定Pax-2基因功能对耳基板诱导、内耳发育和静声神经节(SAG)形成过程中钙粘蛋白表达的影响。