Assembly of the Bacteriophage T4 Primosome

噬菌体 T4 引发体的组装

• 批准号: 6790434
• 负责人: Michelle Marie Spiering
• 金额: $ 4.3万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6790434
• 关键词: DNA binding protein; DNA footprinting; DNA replication; bacteriophage T4; cell line; electron microscopy; fluorescence resonance energy transfer; postdoctoral investigator; protein protein interaction; protein structure function; protein transport; virus assembly; virus genetics; virus protein

DESCRIPTION (provided by applicant): The bacteriophage T4 replication system is one of the best-studied of the related mechanisms of DNA replication employed by prokaryotes and eukaryotes. This proposal is focused on the assembly and functioning of the primosome in the context of DNA replication. A complete understanding will require studying the individual proteins of the primosome as a large enzyme machine. The assembly process of the primosome will be interrogated by ensemble and single-molecule FRET equilibrium and pre-steady state studies. Additional structural information on the primosome will be gained by electron microscopy. The functioning of the primosome will by elucidated by cross-linking/footprinting studies and by monitoring protein-protein interactions using FRET during active translocation and priming by the primosome. Completion of this project will result in a description of the assembly and functioning of the primosome in detail similar to that already determined for the polymerase holoenzyme. Since the process of DNA replication has profound implications for human disease, including cancer, understanding the fidelity of DNA replication is of significant humanitarian and economic interest.

描述（由申请人提供）：