Molecular Mechanisms in a Stem Cell Supporting Niche

干细胞支持生态位的分子机制

• 批准号: 6737870
• 负责人: LI LIN
• 金额: $ 1.68万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2004-04-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6737870
• 关键词: bioinformatics; cell line; flow cytometry; gene expression; gene targeting; hematopoietic stem cells; laboratory mouse; microarray technology; phenotype; polymerase chain reaction; postdoctoral investigator; protein structure function

DESCRIPTION (provided by applicant): The proposed research seeks to identify molecules whose expression is essential in cells that constitute the stem cell niche in the hematopoietic microenvironment. We are using stromal cell lines that have been shown to be highly supportive for stem cells as surrogates for the in vivo niche. The gene profiles of these stromal cell lines which are from different developmental and tissue origin will be compared with those of less-supportive lines by microarray analyses and verified by quantitative real time PCR. Candidate genes that correlate with a stem cell supporting phenotype will be analyzed by bioinformatic tools for functional designation and predicted cellular role. A selected set of gene products will be investigated further with gene knock-down and over expression methodologies. This study will contribute to our understanding of the molecular mechanisms that govern the hematopoietic stem cell niche and perhaps stem cell niches in general.

描述（由申请人提供）： 拟议的研究旨在鉴定其表达对于构成造血微环境中干细胞生态位的细胞至关重要的分子。我们正在使用已被证明高度支持干细胞的基质细胞系作为体内生态位的替代品。这些来自不同发育和组织来源的基质细胞系的基因谱将通过微阵列分析与支持性较低的细胞系进行比较，并通过定量实时 PCR 进行验证。 将通过生物信息学工具分析与干细胞支持表型相关的候选基因，以进行功能指定和预测细胞作用。将使用基因敲低和过度表达方法进一步研究一组选定的基因产物。这项研究将有助于我们了解控制造血干细胞生态位以及一般干细胞生态位的分子机制。