Stem cell-based neural tissue engineering through the use of biomaterials.

通过使用生物材料进行基于干细胞的神经组织工程。

• 批准号: 2302243
• 金额: --
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2302243
• 关键词: Stem; cell; based; neural; tissue

There is an urgent unmet clinical need for scaffolds that actively promote theregeneration of neural tissue. This project will aim to develop a novel, 3D degradable scaffoldwith controlled mechanical and chemical properties, recapitulating the natural extracellularmicroenvironment and releasing growth factors (GFs) in a controlled spatiotemporal manner.The scaffold will be based on Poly(ethylene) glycol and extracellular matrix proteins, such aslaminin and fibronectin, presenting high affinity for GFs and therefore required to support theregenerative process. The scaffolds will thus represent a synthetic microenvironment whereneural stem cells can be cultured in 3D and differentiated through mechanical and chemicalqueues to replace lost neurons and surrounding glia. The development of an organotypic cultureof tissue-specific stem cells in a hydrogel will provide a viable vector for delivering new cells andgrowth promoting factors to an injury site. Furthermore, this culture model could potentiallyhave future applications for screening drugs that could enhance neural tissue regeneration bystimulating the expansion of the stem cells in culture.

临床上迫切需要能够促进神经组织再生的支架，但尚未得到满足。该项目旨在开发一种新型的3D可降解支架，具有可控的力学和化学性质，重现自然的细胞外微环境，并以可控的时空方式释放生长因子(GFS)。支架将以聚乙二醇和细胞外基质蛋白(如层粘连蛋白和纤维连接蛋白)为基础，具有对GFS的高亲和力，因此需要支持再生过程。因此，支架将代表一个合成的微环境，在那里可以三维培养神经干细胞，并通过机械和化学队列进行分化，以取代丢失的神经元和周围的神经胶质细胞。水凝胶中组织特异性干细胞器官型培养的发展将为将新细胞和促生长因子输送到损伤部位提供一个可行的载体。此外，该培养模型可能在筛选通过刺激培养中的干细胞扩张来促进神经组织再生的药物方面具有潜在的应用前景。