SPECIALIZED CENTER OF RESEARCH IN HYPERBARIC OXYGEN THER

描述(申请人摘要)：本申请是一个新项目 这代表了一项多学科的努力来研究这种机制 高压氧(HB02)的作用、安全性和临床疗效 心理治疗。HB02 Herapy已被指定为免费或替代 由国家补充和替代医学中心提供的医疗模式。 该计划包括4个研究项目和3个支持核心，它们是 中心假设：HB02疗法将使头颈癌受益 放射治疗后必须接受重建手术的病人 因为，在药物剂量下，氧气促进血管生成并阻碍 特定类型的细胞间黏附。四个主题中的共同主题 研究项目是生物和临床对海拔高度的反应 环境中头颈部肿瘤、内皮细胞、巨噬细胞和 中性粒细胞。项目1将开发一个手术风险预测模型， 因癌症需要喉切除的患者，验证模型并评估 HB02治疗对改善预后的疗效。促进血管生成 在项目1和项目2中都假设了HB02在照射组织中的作用。 在这两个项目中的血管减少将直接通过 活检样本的组织病理学分析和一种新的方法， 依赖于02与硝基咪唑化合物EF-5的组织结合。项目2 将评估HB02对照射小鼠血管生成的作用机制 肿瘤异种移植生长反应。项目3提议高架部分 压力02会减少转移的头颈部肿瘤细胞和 中性粒细胞附着在肺内皮细胞上。项目4建议 HB02增加第二信使一氧化氮的内源性产生 反过来，这可能会在这个项目中研究的许多反应中发挥作用。 科学核心是生物统计和数据管理(核心B)和Chamber和 高压设备(C芯)。整个计划将由一个 行政核心(核心A)。这一协调一致的计划将提供新的 深入了解HB02的作用机制，并评估临床使用的安全性。

DESCRIPTION (Applicant's Abstract): This application is for a new program that represents a multidisciplinary effort to investigate the mechanisms of action, safety and clinical efficacy of Hyperbaric Oxygen (HB02) Therapy. HB02 herapy has been designated a complimentary or alternative modality by the National Center for Complementary and Alternative Medicine. This program consists of 4 Research Projects and 3 Support Cores which are centered on the hypothesis that: HB02 therapy will benefit head and neck cancer patients who must undergo reconstructive surgery after radiation therapy because, at pharmacological doses, oxygen augments angiogenesis and impedes specific types of intercellular adherence. Common themes within the four research projects are the biology and clinical responses to elevations in ambient 02 concentration of head and neck tumors, endothelium, macrophages and neutrophils. Project 1 will develop a predictive model for surgical risk among patients requiring laryngectomy due to cancer, validate the model and assess the efficacy of HB02 therapy for improving outcome. Stimulation of angiogenesis in irradiated tissue by HB02 is hypothesized in both Projects 1 and 2. Hypoxia and hypovascularity in both projects will be directly evaluated by histopathological analysis of biopsy samples and by a novel method, 02-dependent binding to tissues of the nitroimidazole compound EF-5. Project 2 will evaluate the mechanism for angiogenesis by HB02 in irradiated mice and tumor xenograft growth responses. Project 3 proposes that elevated partial pressures of 02 will reduce metastatic head and neck tumor cells and neutrophils from attaching to pulmonary endothelium. Project 4 proposes that HB02 increases the endogenous production of nitric oxide, a second messenger that, in turn, may play a role in many responses studied in this program. Scientific cores are Biostatistics and Data Management (Core B) and Chamber and High Pressure Equipment (Core C). The entire program will be supported by an administrative core (Core A). This coordinated program will provide new insights into mechanisms for HB02 and assess safety for clinical use.