Development of vaccines against anthrax

炭疽疫苗的研制

• 批准号: 6678385
• 负责人: DENNIS J KOPECKO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6678385
• 关键词: Bacillus anthracis; Salmonella typhi; anthrax; anthrax vaccines; bacterial antigens; bacterial genetics; bioterrorism /chemical warfare; genetic promoter element; host organism interaction; laboratory mouse; plasmids; vaccine development; vector vaccine

Summary: This project is aimed at introducing genes encoding key protective antigens of Bacillus anthracis into a safe, attenuated live Salmonella typhi vaccine vector. The anthrax protective antigen gene(s) will be inserted into several plasmid vectors behind assorted promoters that allow for a low or high level of constituitive expression. Alternatively, promoters that "turn on" the anthrax gene(s) only upon infection or only inside host cells will be employed. These plasmid constructs will initially be examined for level of antigen production and genetic stability in S. typhi vaccine vectors. Several selected candidate live, oral Salmonella-based vaccines against anthrax will be assessed for immunogenicity in mice and for protective efficacy in small animal models.

总结：本项目旨在将编码炭疽杆菌关键保护性抗原的基因导入安全的减毒伤寒沙门氏菌活疫苗载体。 将炭疽保护性抗原基因插入到几个质粒载体中，位于允许低或高水平组成型表达的各种启动子后面。或者，将使用仅在感染时或仅在宿主细胞内“打开”炭疽基因的启动子。这些质粒构建体将首先检查抗原产生水平和在S.伤寒疫苗载体。将评估几种选定的抗炭疽沙门氏菌口服活疫苗在小鼠中的免疫原性和在小动物模型中的保护效力。