Cryoprobe for 800 MHz NMR Spectrometer

用于 800 MHz NMR 波谱仪的冷冻探针

• 批准号: 6732309
• 负责人: MARK A RANCE
• 金额: $ 34.54万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732309
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cryoscopy; macromolecule; nuclear magnetic resonance spectroscopy

The recent development of cryoprobe technology is providing tremendous gains in the inherent sensitivity of solution-state NMR spectrometers. This achievement is of enormous value for NMR studies in the field of structural biology, due to the fundamental role of instrument sensitivity in determining the feasibility of most biomolecular NMR research proposals. Current cryoprobe technology has allowed sensitivity improvements by factors in the range of 2 to 4 to be realized, with the exact gain depending on the conductivity of the sample buffer. In contrast, improvements in sensitivity of conventional NMR probes by as little as 20% have been exceptionally difficult to achieve, and would be considered quite significant. Utilization of a cryoprobe allows sample concentrations to be reduced by factors of 2-4, which greatly facilitates work on samples with low solubility, a tendency to aggregate, or in cases where the sample material is especially difficult and/or costly to obtain. Alternatively, it is possible, by using a cryoprobe, to reduce total data acquisition times by factors of 4 to 16, which is extremely valuable when working on samples of limited stability, when considering projects which require extensive amounts of data to be collected, for allowing for the possibility of performing low-sensitivity experiments in realistic periods of time, and for significantly improving the overall throughput on very costly, ultra-high field NMR spectrometers. In order for structural biologists to continue to tackle increasingly difficult problems, it is vital to exploit whatever technological advances are available. The current proposal requests funds to purchase a triple resonance 5 mm1H-{13C,15N} pulsed field gradient cryoprobe and required accessories for the 800 MHz NMR spectrometer that is housed in the structural biology/NMR facility within the University of Cincinnati College of Medicine. This facility supports a large number of NIH-funded investigators, and acquisition of the cryoprobe will greatly facilitate the existing research projects and will allow new and even more challenging problems to be tackled.

低温探针技术的最新发展为溶液态核磁共振光谱仪的固有灵敏度提供了巨大的进步。这一成就对于结构生物学领域的核磁共振研究具有巨大的价值，因为仪器灵敏度在确定大多数生物分子核磁共振研究方案的可行性方面起着重要作用。目前的低温探针技术已经允许在2到4的范围内实现灵敏度的提高，精确的增益取决于样品缓冲液的导电性。相比之下，将传统核磁共振探针的灵敏度提高20%是非常困难的，并且被认为是相当重要的。低温探针的使用允许样品浓度降低2-4倍，这极大地促进了对溶解度低的样品的工作，倾向于聚集，或者在样品材料特别困难和/或昂贵的情况下获得。或者，通过使用冷冻探针，可以将数据采集时间减少4到16倍，这在处理稳定性有限的样品时，在考虑需要收集大量数据的项目时，在允许在实际时间内进行低灵敏度实验的可能性时，以及在显着提高灵敏度时，都是非常有价值的