Typhoon 9410 Gel/Blot Imager

描述(由申请人提供)：申请资金购买新的台风9410凝胶/斑点成像仪，用于EVMS，安装在微生物学和分子细胞生物学系的生物医学蛋白质组学中心。核心设施将服务于整个EVMS园区。主要用户是7名NIH资助的研究人员和3名NIH资助的调查人员，他们共同代表了EVMS的4个部门。其中，主要用户拥有9笔NIH拨款。首席调查员塞梅斯博士于2000年从弗吉尼亚大学移居EVMS。他目前是微生物学和分子细胞生物学系的副教授和EVMS生物医学蛋白质组学中心的研究主任。塞梅斯博士在使用各种定量分子方法来确定蛋白质、DNA、RNA的大分子相互作用方面拥有超过12年的经验。9410号台风将对他在分子病毒学和癌症蛋白质组学方面的研究做出重大贡献。Forgacs女士和Hanson博士将负责管理台风号的日常操作和培训要求。Forgacs女士和Hanson博士在蛋白质和核酸定量方面拥有五年以上的研究技术经验。请求使用这一仪器的主要原因是为了便利主要用户群体的研究以及今后几年将雇用的新调查员的研究。EVMS现有的磷成像仪不足以支持这项提案中概述的实验，也不足以支持NIH未来的研究努力。为了维持和扩大主要用户群体和其他群体的NIH资金，有必要用这种新的最先进的磷成像仪取代目前陈旧的磷成像仪，这将增强NIH资助的研究人员的分子检测方法。此外，几名调查人员将能够利用台风的特征进行新的调查。

DESCRIPTION (provided by applicant): Funds are requested to purchase a new Typhoon 9410 Gel/Blot Imager for EVMS to be housed in the Center for Biomedical Proteomics in the Department of Microbiology and Molecular Cell Biology. The core facility will serve the entire EVMS campus. Major users are 7 NIH-funded investigators and 3 investigators with NIH funding either pending or under evaluation; together they represent 4 departments at EVMS. Between them, the major users have 9 NIH grants. The Principal Investigator, Dr. Semmes, moved to EVMS from the University of Virginia in 2000. He is currently an Associate Professor in the Department of Microbiology and Molecular Cell Biology and the Research Director of the EVMS Center for Biomedical Proteomics. Dr. Semmes has over twelve years of experience in using a variety of quantitative molecular approaches to determining macromolecular interactions of protein, DNA, RNA. The Typhoon 9410 will contribute significantly to his research efforts in molecular virology and cancer proteomics. Ms. Forgacs and Dr. Hanson, will be the individuals responsible for managing the day-to-day operation and training requirements of the Typhoon. Ms. Forgacs and Dr. Hanson have over five years of research technical experience in protein and nucleic acid quantitation. The major reason for requesting this instrument is to facilitate the research of the major user group and that of new investigators who will be hired over the next several years. The existing phosphorimager at EVMS is inadequate to support the experiments outlined in this proposal and to support future NIH research endeavors. In order to maintain and expand the NIHfunding of the major user group and others, it is necessary to replace the current antiquated phosphorimager with this new state-of-the-art phosphorimager, which will augment the molecular detection methods for the NIH funded investigators. Additionally, several investigators will be able to undertake new avenues of investigation using the Typhoon's features.