JEOL 1230 TEM and BOC Edwards Vacuum Evaporator

描述（由申请人提供）：本提案申请JEOL 1230透射电子显微镜（TEM）和BOC Edwards Auto306真空蒸发器的资金。这些仪器将位于爱荷华大学中央显微镜研究设施。JEOL 1230是1987年用大学资金购买的日立H-7000，目前只有一台TEM。在过去的12个月里，有78个学院实验室使用了该仪器来支持他们的研究。这些调查人员中有60人获得了NIH的研究资助。每学期有8名学生参加电子显微镜技术课程并使用该仪器。调查人员必须提前2到3周报名参加日立TEM。许多作业者在晚上和周末使用TEM。由于意外停机进行维护，无法保证仪器在预定时间内正常工作。这是由于非常频繁的使用，日立的年龄，这是1981年推出的设计，以及服务人员的专业知识水平低的结合。研究人员还需要低剂量成像、自动阶段控制、断层扫描、数字成像和低温透射电镜来研究他们的样品。日立TEM缺乏所有这些现代功能。中银爱德华兹真空蒸发器将取代工厂中已有30年历史的瓦里安机组。维修零件已超过15年；它的缺点是真空度差，碳和金属的蒸发不一致。用于薄切片的碳涂层网格和用于负染色、金属涂层和低温透射电镜的碳薄膜非常薄且对比度低，这一点至关重要。JEOL TEM和BOC Edwards蒸发器将分别位于Eckstein医学研究大楼的84室和92室，作为爱荷华大学中央显微镜研究设施的一部分。设施内经验丰富的人员将提供管理和维修以及高级技术援助和培训。机构支持是强有力的，为购买仪器提供了61,234美元，为研究助理提供了55,133美元的全薪支持，并为JEOL TEM的年度服务合同提供了10,000.00美元的部分资金。威尔士将研究气道上皮细胞生物学，气道感染和基因转移。Apicella计划调查细菌的发病机制。戴利将检查突触发育和大脑损伤。麦克雷将参与使用慢病毒载体的基因转移。Tobacman将研究肌动蛋白短丝的结构。Zabner将检查腺相关病毒的形态。

DESCRIPTION (provided by applicant): This proposal requests funds for a JEOL 1230 Transmission Electron Microscope (TEM) and a BOC Edwards Auto306 Vacuum Evaporator. These instruments will be located in the University of Iowa Central Microscopy Research Facility. The JEOL 1230 is being requested because the facility currently has just one TEM, a Hitachi H-7000 purchased with university funds in 1987. During the past 12 months, 78 faculty labs have used this instrument to support their research. 60 of these investigators have NIH funded research grants. 8 students each semester are enrolled in an Electron Microscopy Techniques course and use this instrument. Investigators have to sign up for the Hitachi TEM 2 to 3 weeks in advance. A number of operators are using the TEM during evenings and weekends. There is no guarantee that the instrument will be functional at a scheduled period because of unexpected downtime for maintenance. This results from a combination of very heavy use, the age of the Hitachi, which is a design introduced in 1981, and the low level of expertise of service personnel. Investigators also need low dose imaging, automated stage controls, tomography, digital imaging and Cryo-TEM to study their samples. The Hitachi TEM lacks all of these modern capabilities. The BOC Edwards Vacuum Evaporator will replace a 30 year old Varian unit in the facility. Repair parts have been unavailable for over 15 years; it suffers from poor vacuum and inconsistent evaporation of carbon and metals. It is critical that carbon coated grids for thin-sections and carbon thin films for negative staining, metal coating and Cryo-TEM be very-thin and low contrast. The JEOL TEM and the BOC Edwards evaporator will be conveniently located in room 84 and 92 respectively in the Eckstein Medical research Building as part of the University of Iowa Central Microscopy Research Facility. Administration and maintenance, as well as high-level technical assistance and training, will be provided by experienced personnel within the facility. Institutional support is strong with $61,234.00 being provided towards purchase of the instruments, $55,133.00 in full salary support for a research assistant and $10,000.00 to partially fund the annual service contract for the JEOL TEM on a continuing basis. Welsh will study airway epithelial cell biology, airway infections and gene transfer. Apicella plans to investigate the pathogenesis of bacteria. Dailey will examine synapse development and injury in brain. McCray will be involved with gene transfer using lentiviral vectors. Tobacman will study the structure of short actin filaments. Zabner will examine the morphology of Adeno-associated virus.