Transmission Electron Microscopy Instrumentation

透射电子显微镜仪器

• 批准号: 6730833
• 负责人: MARK S MOOSEKER
• 金额: $ 38.01万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730833
• 关键词: biochemistry; biomedical equipment purchase; cytoskeleton; organelles; transmission electron microscopy

DESCRIPTION (provided by applicant): Ultrastructural analyses using a variety of experimental approaches are central to ongoing and proposed studies within the laboratories of the primary user groups participating in this shared instrumentation proposal. The primary user group consists of five, highly interactive, NIH-funded laboratories (Mooseker, Forscher, Pollard, Rosenbaum and Wells), all of which are members of the Department of Molecular, Cellular and Developmental Biology (MCDB), Yale University. The long term objective of this proposal is to upgrade/replace the transmission electron microscopy (TEM) equipment currently housed in the MCDB EM facility. This well established facility, maintained and operated by a full time, highly skilled EM technician/instructor, provides both research and training support to the research laboratories in MCDB. The principal investigator and four co-investigators represent the current major research groups using this facility, although a number of other laboratories from several departments also use this facility. Unfortunately, the existing TEMs and ancillary support equipment in this facility are quite old and maintenance due to lack of replacement parts will soon render this facility inoperable. As a consequence, the NIH-funded research projects of the user group, all of which employ ultrastructural analyses, are in jeopardy. The research goals of the user group all focus on structural, biochemical and functional analyses of a wide range of components of the eukaryotic cell cytoskeleton. TEM is used for a broad scope of investigations, from ultrastructural studies on purified cytoskeletal (both actin-filament and microtubule associated) components, isolated organelles, cells and tissues, including cells with altered cytoskeletal functions resulting from mutations in various cytoskeletal components. The requested TEM equipment is essential for the research objectives of the user group, and will also provide much needed research support for the broader research community at Yale.

描述（由申请人提供）：使用各种实验方法的超微结构分析是参与此共享仪器提案的主要用户组实验室中正在进行和拟议研究的核心。主要用户组由5个高度互动的、由美国国立卫生研究院资助的实验室组成（Mooseker、Forscher、Pollard、Rosenbaum和Wells），它们都是耶鲁大学分子、细胞和发育生物学（MCDB）部门的成员。该提案的长期目标是升级/更换目前安装在MCDB EM设施中的透射电子显微镜（TEM）设备。这个完善的设施，由全职，高技能的EM技术人员/讲师维护和操作，为MCDB的研究实验室提供研究和培训支持。首席研究员和四名共同研究员代表了目前使用该设施的主要研究小组，尽管来自几个部门的许多其他实验室也使用该设施。不幸的是，该设施现有的tem和辅助支持设备相当旧，由于缺乏更换部件，维修将很快使该设施无法使用。因此，美国国立卫生研究院资助的用户组研究项目，所有使用超微结构分析的项目，都处于危险之中。用户组的研究目标都集中在真核细胞骨架的广泛组成部分的结构，生化和功能分析。透射电镜用于广泛的研究，从纯化的细胞骨架（肌动蛋白丝和微管相关）成分的超微结构研究，分离的细胞器，细胞和组织，包括因各种细胞骨架成分突变而导致细胞骨架功能改变的细胞。所要求的TEM设备对于用户组的研究目标至关重要，并且还将为耶鲁大学更广泛的研究社区提供急需的研究支持。