JEOL Transmission Electron Microscope

描述（由申请人提供）：这项共享设备资助的目的是为加州大学旧金山分校（UCSF） J. David Gladstone研究所的生物医学研究人员获得JEOL JEM-1230透射电子显微镜，标本旋转支架，GATAN Ultrascan 1000 CCD相机和专用计算机。新设备的用户拥有广泛的美国国立卫生研究院支持的生物医学研究项目，并共同拥有数十年的电子显微镜经验。他们的兴趣包括研究核膜的遗传和获得性缺陷，探索不同载脂蛋白E亚型在神经退行性疾病中的作用，确定G蛋白对心肌病发展的影响，以及研究甘油三酯合成基因在肝脏和肠道中的作用。目前，对电子显微镜的需求远远超过了我们的能力。这种新型显微镜具有数字成像能力，将大大加快获取超微结构图像的速度，从而有可能解决更多的研究问题。此外，与旧仪器不同，新仪器更容易使用，除了专业电子显微镜专家之外，新手也可以使用。新的电子显微镜核心设备将由Stephen G. Young博士管理。日常工作将由拥有30年连续电子显微镜研究经验的王金妮女士监督。罗伯特·汉密尔顿博士（UCSF解剖学教授），一位杰出的电子显微镜专家，将在技术和组织问题上为核心提供咨询。格拉德斯通致力于仪器的维护，并为核心员工提供工资支持。新型电子显微镜对格拉德斯通研究人员培养新一代生物医学研究人员的能力至关重要。生物医学研究问题的答案越来越需要生物化学和超微结构方法的结合。新的电子显微镜将使我们能够向学员传授多学科的生物医学研究方法。

DESCRIPTION (provided by applicant): The objective of this shared equipment grant is to obtain a JEOL JEM-1230 transmission electron microscope, a specimen rotation holder, a GATAN Ultrascan 1000 CCD camera, and a dedicated computer, for the community of biomedical investigators at the J. David Gladstone Institutes at the University of California, San Francisco (UCSF). The users of the new equipment have a wide range of NIH-supported biomedical research projects and collectively boast many decades of experience with electron microscopy. Their interests include investigating genetic and acquired defects in the nuclear envelope, exploring the roles of different apolipoprotein E isoforms in neurodegenerative disease, defining the influence of G proteins on the development of cardiomyopathy, and studying the roles of triglyceride-synthesizing genes in the liver and intestine. Currently, the demand for electron microscopy far exceeds our capacity. The new microscope, with its digital imaging capabilities, will greatly accelerate the acquisition of ultrastructural images, making it possible to tackle many more research problems. Moreover, the new instrument, unlike older instruments, is much easier to use, opening the door to use by novices, in addition to professional electron microscopists. The new Electron Microscopy Core Facility will be run by Dr. Stephen G. Young. Day-to-day operations will be supervised by Ms. Jinny Wong, who has 30 years of continuous electron microscopy research experience. Dr. Robert Hamilton (UCSF professor of anatomy), a distinguished electron microscopist, will consult with the Core on technical and organizational issues. Gladstone is committed to the maintenance of the instruments and for providing salary support for the Core staff. The new electron microscope is absolutely critical to the ability of Gladstone investigators to train a new generation of biomedical investigators. Increasingly, the answers to biomedical research problems require a combination of biochemical and ultrastructural approaches. The new electron microscope will make it possible for us to teach our trainees a multidisciplinary approach to biomedical investigation.