LCQ Deca-XP Ultra Sensitive Ion Trap Mass Spectrometer

描述（由申请人提供）：12名NIH支持的研究者申请资金购买高灵敏度离子阱质谱仪系统，包括二维LC、电喷雾电离、数据依赖采集以及蛋白质/肽鉴定和测量软件。该仪器将生成用于蛋白质鉴定和定位翻译后修饰位点的肽序列信息，以及用于蛋白质组表征的定量数据，作为四个主要用户和八个次要用户的细胞状态的函数。该仪器将位于生物分析和生物技术实验室，2单元，WSU的核心MS设施。该仪器支持的研究项目包括鉴定与小鼠突变株的睡眠反应相关的蛋白质/肽，精子发生期间体细胞和生殖细胞中的基因表达，原始卵泡发育，细胞-细胞相互作用和睾丸发育，以及肌联蛋白激酶结构域在肌肉功能和疾病中的作用。

DESCRIPTION (provided by applicant): Twelve NIH supported investigators request funds to purchase a high sensitivity ion trap mass spectrometer system, including two dimensional LC, electro spray ionization, data dependent acquisition, and protein/peptide identification and measurement software. The instrument will generate peptide sequence information for protein identification and for locating sites of posttranslational modification, and quantitative data for proteome characterization as a function of cell state for four major users and eight minor users. The instrument will be located in the Laboratory for Bioanalysis and Biotechnology, Unit 2, WSU's core MS facility. Research projects to be supported by this instrument include identifying proteins/peptides associated with sleep responses in a mutant strain of mouse, gene expression in somatic and germ cells during spermatogenesis, primordial follicle development, cell-cell interactions and testis development, and the role of titin's kinase domain in muscle function and disease.