XL-I Analytical Ultracentrifuge and Fluorescence Optics

描述（由申请人提供）：大分子相互作用机构，也称为“Mac-In-Fac”（网址：macinfac.bio.unc.edu），是查佩尔山的北卡罗来纳州大学的核心机构。该设施自1996年以来一直在运作。该设施拥有Biacore X、Biacore 2000、配备扫描UV/维斯光学器件的Beckman Optima XL-A分析超速离心机、荧光分光光度计、差示扫描和等温滴定量热仪、圆二色性分光光度计以及静态和动态光散射仪器。有没有类似的设施，在校园查佩尔山，或附近的机构。它目前的用户来自11个部门在北查佩尔山校园（生物化学和生物物理学，生物学，细胞生物学，化学，微生物学和免疫学，病理学，儿科学，药理学，药学，物理学和生理学），其他学术和研究机构从北卡罗来纳州和几个研究三角公园生物技术公司。我们的许多用户在使用Optima XL-A分析超吸收仪的吸光度光学器件进行项目时遇到了问题。这些和其他项目需要使用Optima XL-I提供的干涉光学器件进行。此外，其他一些用户需要具有基于荧光光学的检测系统的分析超级仪器（请参见所附的具体研究计划）。该地理区域（Chapel Hili-Raleigh-达勒姆- RTP）没有此类仪器。因此，Mac-ln-Fac申请资金用于：（1）购买基于干涉和吸光度光学的分析超灵敏度仪（Beckman Optima XL-I），以及（2）在我们现有的Optima XL-A中购买和安装荧光光学检测系统（该附件将由新罕布什尔州大学高级分子相互作用科学中心（CAMIS）制造和安装）。

DESCRIPTION (provided by applicant): The UNC Macromolecular Interactions Facility, also called "Mac-In-Fac" (web address: macinfac.bio.unc.edu), is a core facility of the University of North Carolina at Chapel Hill. The facility has been operating since 1996. The facility has a Biacore X, a Biacore 2000, a Beckman Optima XL-A analytical ultracentrifuge with scanning UV/VIS optics, a fluorescence spectrophotometer, differential scanning and isothermal titration calorimeters, circular dichroism spectropolarimeters, and static and dynamic light scattering instruments. There are no similar facilities on the UNC Chapel Hill Campus, or nearby institutions. Its current users come from 11 departments on the UNC Chapel Hill campus (Biochemistry & Biophysics, Biology, Cell Biology, Chemistry, Microbiology & Immunology, Pathology, Pediatrics, Pharmacology, Pharmacy, Physics, and Physiology), other academic and research institutions from North Carolina and several Research Triangle Park biotechnology companies. Many of our users have experienced problems while carrying out their projects using the absorbance optics available with the Optima XL-A analytical ultracentrifuge. These and other projects need to be carried out with the interference optics available with the Optima XL-I. Additionally, some other users have a need for an analytical ultracentrifuge with a fluorescence optics-based detection system (please see attached specific research plans). No such instrumentation is available in this geographical area (Chapel Hili-Raleigh-Durham- RTP). Therefore, the Mac-ln-Fac requests funds for: (1) purchasing an interference and absorbance optics based analytical ultracentrifuge (Beckman Optima XL-I), and (2) purchasing and installing a fluorescence optical detection system in our existing Optima XL-A (this accessory will be manufatured and installed by The Center to Advance Molecular Interaction Sciences (CAMIS), University of New Hampshire).