Using transposon mutagenesis and high throughput next generation sequencing to identify Staphylococcus aureus genes essential for acid stress survival

医院获得性感染耐甲氧西林金黄色葡萄球菌（MRSA）菌株是疾病和死亡的主要原因，给患者和医院带来了巨大的经济成本。耐甲氧西林金黄色葡萄球菌染色剂现在也成为重要的社区病原体，在以前健康的个体中引起感染，没有可识别的危险因素。需要新型抗生素和更有效的方法来治疗这类感染。金黄色葡萄球菌生长和抗逆性所必需的基因目前正在探索其作为新型药物靶点的价值。作为这项工作的一部分，我们的目标是鉴定并随后表征金黄色葡萄球菌抗酸胁迫所必需的基因。

Hospital-acquired infections with methicillin resistant Staphylococcus aureus (MRSA) strains are a leading cause of illness and death and impose a large economic cost on patients and hospitals. MRSA stains have now also emerged as important community pathogens causing infection in previously healthy individuals with no recognizable risk factors. Novel classes of antibiotics and more effective ways of treating such infections are needed. Genes essential for the growth and stress resistance of S. aureus are currently explored for their value as novel drug targets. As part of this work, we aim to identify and subsequently characterize genes that are essential for acid stress resistance in S. aureus.