Using transposon mutagenesis and high throughput next generation sequencing to identify Staphylococcus aureus genes essential for acid stress survival

使用转座子诱变和高通量下一代测序来鉴定对酸应激生存至关重要的金黄色葡萄球菌基因

• 批准号: 2367796
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2367796
• 关键词: Using; transposon; mutagenesis; throughput; next

Hospital-acquired infections with methicillin resistant Staphylococcus aureus (MRSA) strains are a leading cause of illness and death and impose a large economic cost on patients and hospitals. MRSA stains have now also emerged as important community pathogens causing infection in previously healthy individuals with no recognizable risk factors. Novel classes of antibiotics and more effective ways of treating such infections are needed. Genes essential for the growth and stress resistance of S. aureus are currently explored for their value as novel drug targets. As part of this work, we aim to identify and subsequently characterize genes that are essential for acid stress resistance in S. aureus.

耐甲氧西林金黄色葡萄球菌（MRSA）菌株的医院获得性感染是疾病和死亡的主要原因，并且给患者和医院带来巨大的经济成本。MRSA菌株现在也成为重要的社区病原体，导致以前健康的个体感染，没有可识别的风险因素。需要新型抗生素和更有效的治疗此类感染的方法。对S.金黄色葡萄球菌目前正在探索其作为新型药物靶点的价值。作为这项工作的一部分，我们的目标是确定和随后表征基因是必不可少的酸胁迫抗性在S。金黄色。