TRANSGENIC MOUSE/ES CELL SHARED RESOURCE

转基因小鼠/ES细胞共享资源

• 批准号: 6990171
• 负责人: HAROLD L MOSES
• 金额: $ 10.21万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-28 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990171
• 关键词: DNA; assistive reproductive technique; biomedical facility; cryopreservation; embryonic stem cell; gene targeting; genetically modified animals; laboratory mouse; method development; microinjections; neoplasm /cancer

The objective of the Transgenic Mouse/ES Cell Shared Resource to investigators production is assist in the and use of transgenic and gene knockout mice. Through an interdisciplinary approach, the Resource has been able to provide a broader array of services than are available elsewhere to a large base of investigators. The Shared Resource focuses on quality control, the increased potential for collegial interactions, and immediate access to individuals with deep knowledge of transgenic mice. Experienced personnel develop new procedures and applications, such as those that facilitate the use of site-specific recombinases to perform tissue-specific gene knockouts. As the technology for genome manipulation continues to expand, the Transgenic Mice/ES Cell Shared Resource is likely to grow with it. Specifically, the Shared Resource offers pronuclear DNA microinjection; ES cell microinjection into blastocysts; assisted reproduction; gene targeting; and conditional knockouts utilizing the Cre or FIp(e) site-specific recombinase system; embryo cryopreservation and long-term storage; and sperm cryopreservation and storage. Dr. Cathleen Pettepher is the Director of the Transgenic Mouse/ES Cell Shared Resource and offers consultation to investigators, reviews and approves requests for gene targeting experiments and DNA or ES cell microinjections, and tracks the efficiency and quality of the services offered. She has a highly-trained staff that helps her serve VICC researchers.

转基因小鼠/ES细胞资源共享对科研人员生产的目的 协助转基因和基因敲除小鼠的开发和使用。通过跨学科的方法，该资源能够为大批调查人员提供比其他地方更广泛的服务。共享资源的重点是质量控制、增加大学互动的可能性，以及立即接触到对转基因小鼠有深入了解的个人。有经验的人员开发新的程序和应用，例如那些促进使用特定部位重组酶来执行组织特定基因敲除的程序和应用。随着基因组操作技术的不断发展，转基因小鼠/胚胎干细胞共享资源很可能也会随之发展。具体地说， Shared Resources提供原核DNA显微注射、ES细胞囊胚显微注射、辅助生殖、基因靶向和利用CRE或FIP(E)位点特异性重组酶系统的有条件敲除；胚胎冷冻保存和长期储存；以及精子冷冻保存和储存。Katleen Pettepher博士是转基因小鼠/胚胎干细胞共享资源中心的主任，为研究人员提供咨询，审查和批准基因靶向实验和DNA或胚胎干细胞显微注射的请求，并跟踪所提供服务的效率和质量。她拥有一支训练有素的员工队伍，帮助她为VICC研究人员提供服务。