New Approaches for in vitro and in silico Modelling of the Oral Microbiome

口腔微生物组体外和计算机模拟的新方法

• 批准号: 2384849
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2384849
• 关键词: New; Approaches; vitro; silico; Modelling

The oral microbiome is an open, natural microbial system which consists of a huge variety of over 700 different species. Bacterial cells colonise different tissue surfaces within the oral environment, such as the tongue, gums and teeth. The composition of this system is in constant flux, with complex and dynamic interactions between bacterial species occurring. The oral microbiome and the interactions within play an important role in dental carries and periodontal disease, where untreated cases affect billions of people globally. The personal care industry (and its developed products), a global multibillion pound business that brings wellbeing to millions of customers across the globe, impinge directly on the human microbiome.The aim of this project is to develop new in vitro and in silico modelling approaches that can be replicated and used in order to understand, quantify and predict functions underpinning the colonisation in the oral microbiome. This would allow for the determination of factors that lead to oral diseases. Specifically, how a selected bacterial pathogen invades a healthy oral microbiome and paves the way for oral dysbiosis and dental caries to develop. The objectives of the research project are:1. Simulate the invasion of the oral microbiome by a bacterial pathogen in vitro.2. Analyse the invasion of this pathogen and the resulting alteration in a selected oral mock community.3. Develop a mathematical model following a bottom-up approach, involving data collected from the laboratory, in order to characterise the factors underpinning the colonisation of the oral microbiome.4. Validate the model using independent high-throughput data, present the importance of the work conducted and model produced.This research project will allow for a greater understanding of the progression from a healthy, non-caries microbiome to a disease ridden one.

口腔微生物组是一个开放的自然微生物系统，由700多种不同的物种组成。细菌细胞在口腔环境中的不同组织表面，如舌头，牙龈和牙齿。该系统的组成是不断变化的，细菌物种之间发生复杂和动态的相互作用。口腔微生物组及其相互作用在牙齿携带和牙周病中起着重要作用，未经治疗的病例影响着全球数十亿人。个人护理行业（及其开发的产品）是一个全球数十亿磅的业务，为地球仪的数百万客户带来福祉，直接影响人类微生物组。该项目的目的是开发新的体外和计算机模拟方法，可以复制和使用，以了解，量化和预测支撑口腔微生物组定植的功能。这将有助于确定导致口腔疾病的因素。具体来说，选定的细菌病原体如何侵入健康的口腔微生物组，并为口腔生态失调和龋齿的发展铺平道路。本课题的研究目标是：1.在体外模拟细菌病原体对口腔微生物组的入侵。2.在选定的口腔模拟群落中分析该病原体的入侵和由此引起的改变.按照自下而上的方法开发数学模型，包括从实验室收集的数据，以确定口腔微生物组定植的基础因素。使用独立的高通量数据对模型进行验证，展示所进行的工作和产生的模型的重要性。该研究项目将使人们更好地了解从健康，非龋齿微生物组到疾病缠身的微生物组的进展。