CORE--ANIMAL MODELS

核心--动物模型

• 批准号: 6990006
• 负责人: JAN M. VAN DEURSEN
• 金额: $ 5.02万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990006
• 关键词: biomedical facility; disease /disorder model; gene targeting; genetic manipulation; genetically modified animals; health science research support; laboratory mouse; model design /development; neoplasm /cancer; phenotype; transfection; transfection /expression vector

Mouse models have become increasingly important tools used to understand the origins and underlying causes of cancer. The completion of the mouse genome project has demonstrated that there is less than 1% difference in the genomes between mice and humans. As the mouse is a well-defined genetic model capable of high throughput phenotypic assessment, the ability to create novel mice through genetic engineering provides the opportunity for the development of unique cancer models. The purpose of the Animal Models Shared Resource is to provide the infrastructure necessary to generate and distribute genetically modified mice for cancer research to all participating MCCC investigators. Mayo Clinic Scottsdale has an outstanding track record in generating transgenic mice and will provide transgene services, while Mayo Clinic Rochester has generated a multitude of gene knockout mice and will provide the gene "knockout" services. Since 1999 14 MCCC members have utilized these resources representing 39% of the total services provided. The two mouse resources have been organized such that investigators have easy access to all services provided irrespective of the Mayo Clinic site from which they conduct their research.

小鼠模型已成为越来越重要的工具，用于了解起源和 癌症的根本原因。小鼠基因组计划的完成证明，小鼠和人类的基因组差异不到1%。由于小鼠是一种明确的遗传模型，能够进行高通量表型评估，因此通过基因工程创造新型小鼠的能力为开发独特的癌症模型提供了机会。 动物模型共享资源的目的是为所有参与的 MCCC 研究人员提供生成和分发用于癌症研究的转基因小鼠所需的基础设施。斯科茨代尔梅奥诊所在培育转基因小鼠方面拥有出色的记录，并将提供转基因服务，而罗切斯特梅奥诊所已培育出多种基因敲除小鼠，并将提供基因“敲除”服务。自 1999 年以来，已有 14 家 MCCC 会员利用了这些资源，占所提供服务总量的 39%。这两种小鼠资源的组织方式使得研究人员可以轻松访问所提供的所有服务，无论他们在梅奥诊所的哪个地点进行研究。