Pet Core Facility

PET和muPET设施核心为烧伤创伤中心提供了应用核成像技术研究组织、细胞和遗传水平现象的能力。该设施核心支持研究，使我们能够研究人类和活体实验动物的广泛生物过程。特别是，这项技术使用了最先进的传统PET摄像机或muPET摄像机，为我们提供了不止一次研究动物的能力，允许每只动物作为自己的对照，并允许随着时间的推移遵循干预策略。此外，同样的研究也可以在正常的人类志愿者和患者中进行无创的直接研究。muPET相机可以在人类和动物中以1毫米或更小的分辨率成像，它是可移动的，可以在重症监护患者仍处于急性疾病状态时对骨骼肌进行成像。高分辨率分子成像方法的进一步发展将使研究人员能够成像相关组织中的基因表达和骨骼肌凋亡，从而更好地了解C57BL/6小鼠和后来的人类免疫炎症宿主反应。PET和muPET设施核心允许在MGH， Shriners医院和MIT的人类受试者核心和动物研究核心内进行复杂的研究。

The PET and muPET Facility Core provides the Burn Trauma Center with the capability to apply nuclear imaging techniques to study phenomena at the tissue, cellular, and genetic levels. The facility core supports research that allows us to study a wide range of biological processes in both humans and living laboratory animals. In particular, this technology using either state-of-the-art conventional PET cameras or a muPET camera provides us with the ability to study an animal more than once, allows each animal to serve as its own control, and allows interventional strategies to be followed over time. Furthermore, identical studies can also be conducted non-invasively directly in normal human volunteers and patients. The muPET camera, which enables imaging at resolutions of 1 mm or less in both humans and animals, is mobile and will make imaging skeletal muscle possible in our intensive care patients while they are still acutely ill. Further development of molecular imaging methodologies with high resolution will enable investigators to image gene expression in relevant tissues and skeletal muscle apoptosis to better understand the immuno-inflammatory host response initially in C57BL/6 mice and later in humans. The PET and muPET Facility Core allows complex studies to be performed within the Human Subjects Core and Animal Research Cores at the MGH, Shriners Hospital, and MIT. The facility core provides the Burn Trauma Center investigators with the capability to apply PET technology to test scientific hypotheses without the need to become PET experts themselves. The PET and muPET Facility Core offers several services to the Burn Trauma Center. (1) Design and development of new physiological imaging agents labeled with 18F, 11C, 13N, or 15O. (2) Routine Production and dispensing of existing and new imaging agents for PET study protocols. (3) Design of imaging and data analysis protocols for new agents. (4) Routine execution of existing imaging protocols and data analyses. (5) Development of new instrumentation for specific applications within the Center (e.g. mu PET camera). (6) Development of new technology for real-time molecular imaging of apoptosis and gene expression. Support is requested in this facility for methodology and development that will be very helpful in all four projects.