Pathways to synthetic bacteriophages for phage therapy: Do tRNAs control host range and virulence?

用于噬菌体治疗的合成噬菌体的途径：tRNA 是否控制宿主范围和毒力？

• 批准号: 2391804
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2391804
• 关键词: Pathways; synthetic; bacteriophages; phage; therapy

In this project, the interaction between bacteriophage and their bacterial hosts will be examined in terms of their tRNA content. The main focus of this project will be the provenance and role of tRNAs in phage in terms of host range and virulence. The tRNA content in host and bacterial genomes will be examined to evaluate whether there is evidence for co-evolution between bacteriophage and their bacterial hosts.This will be complemented with a study on bacteriophage tRNAs and bacterial codons to unveil whether they code for the same or different tRNAs, examining the identities of the tRNAs in question.This project will aim to create a model system using Klebsiella sp. and Bilbo and Aura phage. Once isolated from the environment, Klebsiella strains will be infected with Bilbo and Aura phage. The strains susceptible to only one of the two phage will then be selected. Phage tRNAs will be sequentially knocked out, and their virulence and host range will be compared against their "wild type" counterparts.

在这个项目中，噬菌体和它们的细菌宿主之间的相互作用将根据它们的tRNA含量进行检查。这个项目的主要焦点将是在宿主范围和毒力方面的来源和作用的tRNAs在噬菌体。研究会将检视宿主及细菌基因组中的tRNA含量，以评估噬菌体与其宿主细菌之间是否存在共同进化的证据。此外，亦会研究噬菌体的tRNA及细菌的密码子，以揭示它们所编码的tRNA是否相同或不同。该项目的目标是利用克雷伯氏菌属和比尔博噬菌体以及奥拉噬菌体建立一个模型系统。一旦从环境中分离，克雷伯氏菌菌株将被Bilbo和Aura噬菌体感染。然后选择仅对两种噬菌体之一敏感的菌株。噬菌体tRNA将被依次敲除，它们的毒力和宿主范围将与它们的“野生型”对应物进行比较。