Biochemical and biophysical mechanisms of macrophage migration and nuclear deformation.

巨噬细胞，特别是组织驻留巨噬细胞(TRMS)，通过清除体内平衡中的细胞碎片，在防御病原体入侵和组织修复中起着至关重要的作用。尽管到目前为止，许多研究已经阐明了TRMS的起源和功能，但对巨噬细胞侵袭组织的生化和生物物理机制和动力学，特别是核机制的作用，知之甚少。为了解决文献中的这一空白，我们将利用浆细胞迁移模型来发育果蝇胚胎，同时使用现有的遗传工具来解开迁移过程中的核机制。使用体内和体外方法，将使用共聚焦和双光子显微镜收集数据，我们打算在其中分析核变形及其在受限迁移中的作用。这些见解将阐明脊椎动物巨噬细胞的迁移，这将为创伤愈合、癌症、再生和对感染的免疫反应等治疗策略的进展提供信息。

Macrophages, specifically tissue resident macrophages (TRMs), are vital in defence against pathogen invasion and tissue repair by eliminating cell debris in homeostasis. Although much of research to date has shed light on the origins and function of TRMs, very little known about the biochemical and biophysical mechanisms and kinetics of macrophage invasion of tissues, especially the role of nuclear mechanisms. To address this gap in the literature, we will utilise the plasmatocyte migration model in developing Drosophila embryos alongside established genetic tools to unravel nuclear mechanics during migration. Using both in vivo and ex vivo methods, data will be collected using confocal and two-photon microscopy where we intend to analyse nuclear deformation and its role in confined migration. These insights will shed light on vertebrate macrophage migration which will inform advancements in treatment strategies including wound healing, cancer, regeneration, and immune response to infections.