The role of NKT cells and lipid stimuli in the development of allergic sensitisation

NKT 细胞和脂质刺激在过敏致敏发展中的作用

• 批准号: 2397745
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2397745
• 关键词: role; NKT; cells; lipid; stimuli

This project aims to investigate the influence of lipids from an allergen source to shift towards a Th2 response of allergic sensitisation.Techniques will be developed to isolate and characterise natural killer T (NKT) cells from human blood samples and then co-culture iNKT cells with lipid and/or protein-loaded dendritic cells. The subsequent cytokine response will be measured using assays to determine whether a Th2 response has been produced. These techniques will be applied to allergic and non-allergic blood samples to characterise the lipid-activation profiles of NKT cells in each group.

该项目旨在研究过敏原来源的脂类转变为过敏反应的Th2反应的影响。将开发从人类血液样本中分离和鉴定自然杀伤T细胞(NKT)的技术，然后将iNKT细胞与负载脂质和/或蛋白质的树突状细胞共同培养。随后的细胞因子反应将通过检测来确定是否已经产生了Th2反应。这些技术将应用于过敏性和非过敏性血液样本，以表征每组NKT细胞的脂质激活特征。