Transcription Regulation and Biological Function of NAG1

NAG1的转录调控及生物学功能

• 批准号: 6799937
• 负责人: SEUNG Joon BAEK
• 金额: $ 10.8万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-08 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6799937
• 关键词: antiinflammatory agents; apoptosis; carcinogenesis inhibitor; chemoprevention; gene expression; genetic promoter element; genetic transcription; genetically modified animals; laboratory mouse; nonsteroidal antiinflammatory agent; recombinant proteins

DESCRIPTION (provided by applicant) Nonsteroidal antiinflammatory drugs (NSAlDs) have been used for the treatment of inflammatory disease. NSAlDs also have anti-tumorigenic activity. An anti-tumorigenic protein, called NSAID activated gene-1 (NAG-1), mediates one molecular mechanism for NSAID-induced anti-tumorigenic activity. It is possible that NAG-1 may contribute to anti-tumorigenic and anti-inflammatory activity produced by NSAlDs. Therefore, the elucidation of transcriptional regulation and the biological functions provides a novel mechanism to better understand cancer and inflammation. The research objectives are to study how NSAlDs regulate NAG-1 gene expression, and also how environmental factors may affect-NAG-1 gene regulation to prevent cancer and inflammation. Two specific aims are involved in this research. First, the promoter of NAG-1 gene will be used to elucidate its NSAID responsiveness and then cloning the NSAID responsive binding protein. Secondly, the biological function of NAG-1 will be assessed using purified recombinant NAG-1 protein and NAG-1 overexpressing transgenic mice, to elucidate its anti-tumorigenic and anti-inflammatory activities. This proposal also seeks to address how NSAlDs regulate anti-tumorigenesis, and whether other factors, believed to modulate tumor development, could do so by altering gene regulation. This study may also provide new insights into how chemical and environmental factors influence cancer development and lead to the development of novel family anti-tumorigenic compounds, which are not cyclooxygenase (COX) inhibitors. Cancer and inflammation are certainly environmentally relevant problems and thus this project will provide new clues to aid in the understanding of how these drugs alter tumor development.

描述（由申请人提供）