Determining the origins of Candida auris from experimental, epidemiological and ecological investigations

通过实验、流行病学和生态调查确定耳念珠菌的起源

• 批准号: 2399257
• 金额: --
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2399257
• 关键词: Determining; origins; Candida; auris; experimental

Fungi are a growing threat to the health of humans. Many fungal diseases of humans are also becoming increasingly difficult to treat with antifungals as pathogens evolve resistance. Candida auris has recently emerged as a multidrug-resistant pathogen of humans that causes severe illness in hospitalised patients. Genetic studies have confirmed C. auris includes as many as five genetically divergent populations. However, it is unknown when and where C. auris evolved, and what ecological niche(s) it occupied before 'spilling over' in to human populations. Using a combination of experimental and phylogenetic approaches, this project seeks to answer the evolutionary origins of C. auris. The closest relatives of C. auris have been found in fish, suggesting the clade may have evolved in an aquatic environment, possibly as a coloniser or pathogen of other fish. Furthermore, C. auris is able to grow at very high temperates and in high salt concentrations. The project will explore ancestral and evolutionary history from whole genome sequence data, as well as the ability of C. auris to colonise the eggs and embryos of Killifish: a new experimental system developed at the University of Exeter that focusses on a species naturally found in shallow warm subtropical and tropical waters.

真菌对人类的健康构成了日益严重的威胁。随着病原体的发展抗药性，许多人类的真菌疾病也变得越来越难以治疗。念珠菌最近已成为人类的多药耐药性病原体，导致住院患者的严重疾病。遗传学研究证实了Aurisc。包括多达五个遗传分歧的人群。但是，尚不清楚Auris何时何地进化，以及在人类中“溢出”之前占据的生态位。该项目结合了实验和系统发育方法，试图回答Auris的进化起源。在鱼类中发现了最接近的auris亲戚，这表明该进化枝可能已经在水生环境中演变而成，可能是其他鱼类的殖民者或病原体。此外，Auris C. Auris能够在非常高的温度和高盐浓度下生长。该项目将从整个基因组序列数据中探索祖先和进化史，以及Auris在埃克塞特大学开发的一种新实验系统的卵形和胚胎的能力，该系统专注于自然在浅水温暖的亚热带和热带水域中发现的物种。

项目成果

Population genetics and microevolution of clinical Candida glabrata reveals recombinant sequence types and hyper-variation within mitochondrial genomes, virulence genes, and drug targets.

• DOI: 10.1093/genetics/iyac031
• 发表时间: 2022-05-05
• 期刊: GENETICS
• 影响因子: 3.3
• 作者: Helmstetter, Nicolas;Chybowska, Aleksandra D.;Delaney, Christopher;Dantas, Alessandra Da Silva;Gifford, Hugh;Wacker, Theresa;Munro, Carol;Warris, Adilia;Jones, Brian;Cuomo, Christina A.;Wilson, Duncan;Ramage, Gordon;Farrer, Rhys A.
• 通讯作者: Farrer, Rhys A.