TECHNOLOGY FOR AUTOMATED ANALYSIS OF PROTEOMES

目前生物学研究中最引人注目的发展之一是从单一基因和蛋白质的分析转向对生物系统和途径的综合分析。这一转变是自动化、高通量基因组技术发展的结果，这些技术应用于对完整基因组进行测序，并在全基因组范围内测量基因表达。目前，还没有可以在蛋白质水平上对生物系统进行分析的强大技术。然而，蛋白质是最重要的一类生物效应分子，如果不了解所涉及的蛋白质的识别、功能和活性状态，就不可能建立一个完整的生物过程模型。本申请中提出的工作旨在开发一种快速、灵敏、定量和全面地分析复杂生物样本中的蛋白质表达谱的技术，最终在整个细胞裂解产物中。一旦开发出来，这种技术将成为生物学家试图从生物系统的功能、控制和机制方面解释基因组的线性信息的必要工具。

One of the most dramatic current developments in biological research is the shift from the analysis of single genes and proteins to the comprehensive analysis of biological systems and pathways. This shift is a consequence of the development of automated, high throughput genomic technologies and their application to sequence complete genomes and to measure gene expression on a genome-wide scale. Currently, no comparably powerful technology is available for the analysis of biological systems on the protein level. Howe er, proteins are the most significant class of biological effector molecules, and a complete model of a biological process cannot be established without knowledge of the identify, function and state of activity of the proteins involved. The work proposed in this application is aimed at the development of a technology for the rapid, sensitive, quantitative and comprehensive analysis of protein expression profiles in complex biological samples, ultimately in whole cell lysates. Once developed such a technology will be an essential tool for biologists' attempts to interpret the linear information of genomes in terms of function, control and mechanisms of biological systems.