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Exploring communication mechanisms between fungal pathogens and plant cells

探索真菌病原体与植物细胞之间的通讯机制

基本信息

批准号：
2401519
负责人：
金额：
--
依托单位：
UNIVERSITY OF EXETER
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2020
资助国家：
英国
起止时间：
2020 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2401519
关键词：
Exploring communication mechanisms between fungal

项目摘要

Climatic, environmental, legislative and societal changes led to the emergence of novel crop pathogens and the evolution of existing problematic species. Phytopathogenic species which cause crop plant diseases are annually responsible for the loss of ~15% of total crop yield globally and are therefore a serious threat to global food security. Particularly serious are Fusarium head blight (FHB)/head scab disease caused by cereal infecting Fusaria fungi (www.scabusa.org) and Zymoseptoria tritici infections in wheat crops (Dean (2012) Molecular Plant Pathology 13, 414-430), both will be studied in this PhD project.The main scientific aims of this project are (A) to investigate both the cellular and molecular mechanisms required for the transition of Fusarium graminearum hyphae from apoplastic to plasmodesmatal growth (Brown (2010), Fungal Biology 114, 555-571; Brown (2017) Mol Plant Pathol 18, 1295-1312) and (B) to explore the functional role(s) of specific plasmodesmata associated wheat proteins (Faulkner (2013) PNAS, 110, 9166-9170). To achieve the project aims the student will learn how to use a range of existing tools (fungal reporter strains, wheat, rice and Arabidopsis transformants), established techniques (RNA seq analyses, light/UV/confocal microscopy, Virus Induced Gene Silencing (VIGS) (Lee (2012) Plant Physiology 160, 582-590) and emerging technologies (such as genome editing). They will also be trained in the use of bespoke software to quantify and mathematically model the in vivo fungal-plant image datasets acquired from their detailed microscopy studies.

气候、环境、立法和社会的变化导致了新的作物病原体的出现和现有问题物种的进化。引起作物病害的植物病原物种每年造成全球约15%的作物总产量损失，因此对全球粮食安全构成严重威胁。特别严重的是小麦作物中由感染镰刀菌属真菌（Fusaria fungi）（www.example.com）的谷物引起的镰刀菌头枯病（Fusarium head blight，FHB）/头疮痂病（head scab diseasewww.scabusa.org（Dean（2012）Molecular Plant Pathology 13，414-430），两者都将在该博士项目中进行研究。该项目的主要科学目的是（A）研究禾谷镰刀菌菌丝从质外体到胞间连丝生长的转变所需的细胞和分子机制（Brown（2010），Fungal Biology 114，555-571; Brown（2017）Mol Plant Pathol 18，1295-1312）和（B），以探索特异性胞间连丝相关小麦蛋白的功能作用（Faulkner（2013）PNAS，110，9166-9170）。为了实现该项目的目标，学生将学习如何使用一系列现有的工具（真菌报告菌株，小麦，水稻和拟南芥转化体），已建立的技术（RNA测序分析，光/紫外/共聚焦显微镜，病毒诱导的基因沉默（VIGS）（Lee（2012）Plant Physiology 160，582-590）和新兴技术（如基因组编辑）。他们还将接受使用定制软件的培训，以量化和数学建模从详细的显微镜研究中获得的体内真菌植物图像数据集。