Dissecting the function of Wnt signalling in zebrafish neural crest development

剖析 Wnt 信号在斑马鱼神经嵴发育中的功能

• 批准号: 2401640
• 金额: --
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2401640
• 关键词: Dissecting; function; Wnt; signalling; zebrafish

In this project the student will use a broad spectrum of tools to visualize and manipulate Wnt signalling during neural crest development in the zebrafish. The student will use zebrafish larvae to characterize Wnt trafficking in the dorsal roof plate. Based on these results, functional analysis of Wnt ligands and receptors will be carried out. Complementary, the student will use CRISPR/Cas9 technology to generate knock-out lines and analyse the phenotype of cells lacking specific components of the Wnt signalling pathway. This technique will be used to analyse the consequences of loss of Wnt signalling effectors in embryogenesis. In combination with a variety of fluorescence labelling techniques, the student will use long-time in-vivo imaging to study Wnt ligand distribution in the neural crest in the zebrafish larvae. Experimental Methods: The student will learn how to use zebrafish as a model, super-resolution imaging and molecular biology techniques to study gene function during embryogenesis.

在这个项目中，学生将使用广泛的工具来可视化和操纵斑马鱼神经嵴发育过程中的Wnt信号。学生将使用斑马鱼幼虫来表征Wnt在背屋顶板上的贩运。基于这些结果，将对Wnt配体和受体进行功能分析。此外，该学生将使用CRISPR/Cas9技术生成敲除系，并分析缺乏Wnt信号通路特定成分的细胞的表型。这项技术将用于分析胚胎发生中Wnt信号效应物丢失的后果。结合多种荧光标记技术，学生将使用长时间体内成像技术研究Wnt配体在斑马鱼幼体神经嵴中的分布。实验方法：学生将学习如何使用斑马鱼作为模型，超分辨率成像和分子生物学技术来研究胚胎发生过程中的基因功能。

项目成果

Neural Crest: Origin, Migration and Differentiation

神经嵴：起源、迁移和分化

• 发表时间: 2022
• 期刊: eLS, John Wiley & Sons, Ltd (Ed.)
• 作者: Sutton G