Identification of factors affecting mastermind functions

描述（由申请人提供）： Notch（N）信号通路定义了一种进化上保守的细胞相互作用机制，其控制不同发育组织中的许多细胞命运选择。Notch信号传导途径元件的突变与人类病理学相关，证明Notch信号传导在发育过程中发挥重要作用。mastermind（mam）的突变表现出神经源性表型，并显示出与Notch通路组分的广泛遗传相互作用，表明Mastermind在Notch信号通路中起重要作用。尽管与核调节蛋白相似，但Mam的功能及其在Notch信号传导中的作用仍然未知。以果蝇为模型系统，本研究的目标是研究mam的生理作用，并希望在遗传和分子水平上阐明mam在Notch信号通路中的作用机制。建议的具体目标如下： 1.确定影响主谋功能的基因外因素：将进行基因筛选，目的是在发育中的翅膀中分离主谋功能的显性修饰因子。 2.识别和分析摄魂分子相互作用：分子分析将补充特定目标1识别摄魂细胞内效应。

DESCRIPTION (provided by applicant): The Notch (N) signaling pathway defines an evolutionarily conserved cell interaction mechanism that controls a number of cell fate choices in distinct developing tissues. Mutations in Notch signaling pathway elements are associated with human pathology demonstrating the important role Notch signaling performs during development. Mutations in mastermind (mam) exhibit neurogenic phenotypes and display extensive genetic interactions with Notch pathway components suggesting that Mastermind plays an important role in the Notch signaling pathway. Despite the similarity to nuclear regulatory proteins the function of Mam remains unknown, as well as its role in Notch signaling. Using Drosophila melanogaster as a model system, the goal of this proposal is to examine the physiological role of mam and hopefully to elucidate, at the genetic and molecular level, the mechanism by which mam functions within the Notch signaling pathway. The following specific aims are proposed: 1. Identify extragenic factors affecting mastermind function: A genetic screen will be carried out aimed at isolating dominant modifiers of mastermind function in the developing wing. 2. Identify and analyze mastermind molecular interactors: A molecular analysis will complement Specific Aim 1 in identifying intracellular effectors of Mastermind.