Genetics, Lipids, and Responses to Exercise Training

遗传学、血脂和运动训练的反应

• 批准号: 6791114
• 负责人: AMY A Halverstadt
• 金额: $ 4.11万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791114
• 关键词: apolipoproteins; blood lipoprotein metabolism; cardiovascular disorder risk; cholesterol; clinical research; exercise; gene environment interaction; gene expression; genetic mapping; genetic polymorphism; genetic screening; genetic susceptibility; genotype; high density lipoproteins; human middle age (35-64); human old age (65+); human subject; lipase; nuclear magnetic resonance spectroscopy; polymerase chain reaction; postdoctoral investigator

DESCRIPTION (provided by applicant): The proposed study will assess and characterize new genetic variants in LIPH and APOL1 that may relate to plasma lipoprotein lipid levels and their changes with exercise training. The study hypothesis is that genetic variants in LIPH and APOL1, candidate genes for HDL metabolism and composition, will partly explain the variability in plasma HDL levels and their changes with exercise training. The study will employ a standardized exercise training intervention in healthy 50-75 year olds, conduct baseline and final testing that includes NMR measurement of plasma lipoprotein lipids, sequence and screen newly identified candidate genes for HDL metabolism, genotype subjects at these newly identified gene loci, and assess the potential association of identified variants with plasma lipoprotein lipid levels and their responses to exercise training. The applicant will be prepared to perform mechanistic studies, including intermediate phenotype, mRNA expression, and blood protein level studies, to validate any associations found. The two months/yr she spends training with Dr. Eric Hoffman (Research Center for Genetic Medicine at Children's National Medical Center, Washington, DC) will train her to use emerging genetic and biochemical techniques to support her state-of-the-art genetics-exercise physiology research at the University of Maryland.

描述（由申请人提供）：拟议的研究将评估和表征LIPH和APOL1的新遗传变异，这些变异可能与血浆脂蛋白脂水平及其随运动训练的变化有关。该研究的假设是，LIPH和APOL1 （HDL代谢和组成的候选基因）的遗传变异将部分解释血浆HDL水平的变异性及其随运动训练的变化。该研究将在50-75岁的健康人群中采用标准化的运动训练干预，进行基线和最终测试，包括血浆脂蛋白脂的核磁共振测量，对新发现的高密度脂蛋白代谢候选基因进行测序和筛选，对这些新发现的基因位点进行基因分型，并评估已发现的变异与血浆脂蛋白脂水平及其对运动训练的反应的潜在关联。申请人将准备进行机制研究，包括中间表型，mRNA表达和血液蛋白水平研究，以验证发现的任何关联。