Core--Skin Analysis and Epidermal Engineering

皮肤分析和表皮工程核心的开发旨在通过促进访问和提供几个重要领域的专业知识来提高本P01中所有项目的生产力。核心将提供最先进的包埋，切片（冷冻或石蜡包埋），常规染色，免疫染色，并使用逆转录病毒构建体，以及时和具有成本效益的方式创建LRC。 不仅传统的免疫标记物可用，但尖端技术与更高的 敏感性和途径特异性免疫探针，旨在定义：凋亡标志物，信号 还将使用转导途径和增殖/分化途径。 在这个核心执行的所有工作将由一个经验丰富的团队进行，具有卓越和生产力的跟踪记录。这个核心的成员和项目之间的合作互动可能每天都会发生。由于Nickoloff博士过去曾担任其他相关核心的核心主任，例如密歇根大学的免疫病理学核心和Loyola大学的SCID小鼠核心，这种专业知识加上芝加哥和安阿伯之间正在进行的高度转化的联合研究工作将确保该核心的成功。 这些服务将包括： 1.提供来自人和鼠组织的皮肤活检标本的专业处理，包括冷冻保存和福尔马林固定。 2.提供常规染色（苏木精和伊红）以及冻存和石蜡包埋切片的免疫组织化学染色。 3.提供所有染色切片的专家皮肤病理学报告，并对所有形态学分析进行定量和半定量测量。 4.制备表征良好的逆转录病毒构建体，用于转导至将用于体外研究和皮肤移植实验的表皮等同物（EE）和Loyola筏培养物（LRC）中。

The Skin Analysis and Epidermal Engineering Core was developed to enhance the productivity of all projects within this P01 by facilitating access and providing expertise in several important areas. The core will render state-of-the art embedding, sectioning (frozen or paraffin-embedded), routine staining, immunostaining, and creation of LRCs using retroviral constructs, in a timely and cost-efficient manner. Not only will conventional immunomarkers be available, but cutting-edge techniques with higher sensitivities and pathway specific immunoprobes aimed at defining: apoptosis markers, signal transduction pathways, and proliferation/differentiation pathways will also be used. All of the work performed in this core will be conducted by an experienced team with a track record of excellence and productivity. Cooperative interaction amongst members of this core and the projects will likely occur on a daily basis. Since Dr. Nickoloff has served as the Core Director for other relevant cores in the past such as the Immunopathology Core at the University of Michigan and the SCID Mouse Core at Loyola University, this expertise coupled with ongoing highly translational joint research efforts between Chicago and Ann Arbor will ensure the success of this core. The services will include the following: 1. To provide specialized handling of skin biopsy specimens derived from both human and murine tissues including cryopreservation and formalin fixation. 2. To provide routine staining (hematoxylin and eosin), as well as immunohistochemical staining of cryopreserved and paraffin embedded sections. 3. To provide expert dermatopathological reports of all stained sections with quantitative and semiquantitative measurements on all morphological analyses. 4. To prepare well-characterized retroviral constructs for transduction into epidermal equivalents (EEs) and Loyola raft cultures (LRCs) that will be utilized for in-vitro studies and skin graft experiments.