Core--MOLECULAR ANALYSIS AND LOCALIZATION

核心--分子分析与定位

• 批准号: 6990141
• 负责人: RICHARD R VAILLANCOURT
• 金额: $ 7.75万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-19 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990141
• 关键词: SDS polyacrylamide gel electrophoresis; animal colony; biomedical facility; confocal scanning microscopy; histopathology; immunocytochemistry; immunoelectron microscopy; in situ hybridization; integrins; laminin; metalloendopeptidases; molecular oncology; neoplastic process; prostate neoplasms; protein localization; proteomics; transmission electron microscopy

In Core C the previous Core B (Morphology and Morphometrics) and Core C (Automated Combined In Situ Hybridization and Immunohistochemistry Laboratory) have been combined and reorganized under the leadership of a proteomics expert to ensure that the techniques for molecular analysis and Iocalizarion by the three projects are adequately provided throughout the five years of this grant renewal and to direct the duties of the technician hired for this service. This core provides a facility for: 1. Automated in situ hydridization (ISH) and immunohistochemistry (IHC) 2. Confocal laser scanning microscopy, morphometrics 3. Transmission Electron Microscopy (TEM) and immunoelectron microscopy (IEM) 4. Proteomics (one- and two-dimensional SDS-PAGE, SELDI and mass spectrometry)

在核心C中，以前的核心B(形态和形态计量学)和核心C(自动化联合原位杂交和免疫组织化学实验室)在一名蛋白质组专家的领导下进行了合并和重组，以确保在这次赠款续期的五年中，这三个项目的分子分析和定位技术得到充分提供，并指导为这项服务雇用的技术人员的职责。 此核心提供了一种设施，用于： 1.自动原位氢化(ISH)和免疫组化(IHC) 2.共聚焦激光扫描显微镜、形态计量学 3.透射电子显微镜和免疫电子显微镜 4.蛋白质组学(一、二维SDS-PAGE、SELDI和质谱学)