Investigating the role of chromatin remodellers in difficult-to-replicate regions of genomes.

研究染色质重塑剂在基因组难以复制区域中的作用。

• 批准号: 2429800
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2429800
• 关键词: Investigating; role; chromatin; remodellers; difficult

The DNA blueprint that defines the characteristics of eukaryotic organisms is made up of Chromatin; DNA and its histone 'wrapping'. Chromatin is the substrate that the replication machinery must travel through to make a second copy. Dense portions of chromatin, known as heterochromatin, are replicated at different speeds than more open areas. Here we intend to map the regions of the genome most sensitive to loss of particular protein activities that are able to move the histone wrapping and help replication machinery through more difficult regions. These findings are critical to our understanding of how genomes are accurately remade at each cell division.

定义真核生物特征的DNA蓝图由染色质、DNA及其组蛋白“包装”组成。染色质是复制机器进行第二次复制时必须穿过的基质。染色质的密集部分，称为异染色质，以不同的速度复制比更开放的区域。在这里，我们打算绘制基因组中对特定蛋白质活性丧失最敏感的区域，这些蛋白质活性能够移动组蛋白包装并帮助复制机器通过更困难的区域。这些发现对于我们理解基因组在每次细胞分裂时是如何准确地重建的至关重要。