NPO gene function in mammalian intestine

描述（由申请人提供）： nil per os（npo）基因是在申请人的K 08奖的赞助下发现的。它编码斑马鱼消化器官发育所需的保守RNA识别基序（RRM）蛋白。在目前的建议中，我们希望将我们对该基因的研究扩展到哺乳动物。我们已经发现NPO在小鼠胚胎消化道和成年小肠的隐窝中表达。基于这些初步数据和我们在斑马鱼中的工作，我们假设在哺乳动物中，NPO是肠道器官发生和稳态所必需的。为了验证这一想法，我们提出了以下具体目标：目的1：分析的空间和发育的表达模式的NPO在小鼠胚胎和成年胃肠道;目的2：确定的作用，NPO在哺乳动物消化器官的发育和稳态的条件基因打靶在小鼠中。这些研究旨在为器官发育的分子机制提供新的见解，最终可能导致器官衰竭和癌症等疾病的新治疗方法

DESCRIPTION (provided by applicant): The nil per os (npo) gene was discovered under the auspices of the applicant's K08 award. It encodes a conserved RNA recognition motif (RRM) protein required for digestive organ development in zebrafish. In the current proposal we wish to extend our studies of this gene into mammals. We have found that NPO is expressed in the mouse embryonic digestive tract and in the crypts of the adult small intestine. Based on these preliminary data and our work in zebrafish, we hypothesize that in mammals NPO is required for intestinal organogenesis and homeostasis. To test this idea we propose to meet the following specific aims: Aim 1: To analyze the spatial and developmental expression patterns of NPO in the mouse embryo and in the adult gastrointestinal tract; Aim 2: To determine the role of NPO in mammalian digestive organ development and homeostasis by conditional gene targeting in the mouse. These studies are intended to provide new insight into the molecular mechanisms of organ development, which ultimately may lead to new treatments for diseases such as organ failure and cancer