Microscopy of deformation processes in lipid bilayers

描述（由申请人提供）：本文描述的工作采用最先进的显微镜测量（光学和扫描探针）来检查脂质双层中的结构域形成。一个特别关注的焦点是脂质结构对变形的响应。本工作的三个具体目标包括：（1）微管载体解聚后囊泡的灾难性降解（该过程类似于灵长类动物中阿尔茨海默氏病的研究）;（2）囊泡融合中的脂质和流体体积混合，随后是三维成像方法;以及（3）表面支持的脂质双层中蛋白质-蛋白质距离限制对结构域形成和细胞骨架生长模式的影响。

DESCRIPTION (provided by applicant): The work described here employs state-of-the-art microscopy measurements (optical and scanning probe) to examine domain formation in lipid bilayers. A particular focus of interest is in the response of lipid structure to deformation. Three specific targets of the work include: (1) catastrophic degradation of vesicles upon depolymerization of microtubule support (a process which has analogies in studies of Alzheimer's disease in primates); (2) lipid and fluid volume mixing in vesicle fusion followed by three-dimensional imaging methods; and (3) the effects of protein-protein distance constraints in surface-supported lipid bilayers on domain formation and cytoskeletal growth patterns.