PATHOGENESIS OF HELICOBACTER HEPATICUS

肝螺杆菌的发病机制

• 批准号: 6846335
• 负责人: CATHERINE S BECKWITH
• 金额: $ 12.27万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6846335
• 关键词: Helicobacter; bacteria infection mechanism; bacterial genetics; disease /disorder model; gene expression; gene targeting; genetically modified animals; hepatitis; inflammatory bowel diseases; laboratory mouse; liver neoplasms; microarray technology; molecular pathology; virulence

DESCRIPTION (Provided by applicant): Catherine Beckwith, D.V.M, Ph.D., Diplomate, American College of Laboratory Animal Medicine, received her doctoral training with Dr. Lela Riley at the University of Missouri where her research focused on development of diagnostic assays, identification of virulence factors, and characterization of the pathogenesis of rodent Helicobacter infections. With the SERCA, Dr. Beckwith will build upon her background in research and obtain additional training that will allow her to develop into an independently funded research investigator. Dr. Beckwith will train in the rich intellectual environment of SU where Dr. Stanley Falkow, an internationally renowned leader in bacterial pathogenesis research, will mentor her. Dr. Beckwith is co-sponsored by Linda Cork, D.V.M., Ph.D., a veterinary pathologist and the Department Chair of Comparative Medicine, who will ensure that she has the resources and time to carry out her research. Clinical and teaching responsibilities will be only as sufficient to maintain her clinical skills. Dr. Beckwith will conduct an in-depth research project, present her research at national scientific meetings, participate in laboratory meetings, attend specialized courses at Cold Spring Harbor Laboratory, and attend seminars and journal clubs related to bacterial pathogenesis. Dr. Beckwith's research will use a molecular and genetic approach to identify and characterize virulence factors in H. hepaticus, the cause of IBD, chronic active hepatitis, and hepatocellular tumors in mice. The H. hepaticus-infected mouse is an animal model of chronic bacterial infection, which closely mimics human hepatobiliary and inflammatory bowel diseases. Dr. Beckwith will create a transposon library in H. hepaticus and identify virulence factors through negative selection in mice. Novel virulence factors and the host response will be characterized using traditional and cutting edge techniques including global gene expression analysis with microarrays. Characterization of virulence factors is essential to understanding the pathogenesis of IBD and hepatitis caused byH. hepaticus. Recent evidence suggests a link between helicobacters and chronic hepatobiliary diseases in humans. The long-term goal of the proposal is to develop new strategies for prevention and treatment of chronic liver and intestinal disease in humans.

描述(由申请人提供)：Catherine Beckwith，D.V.M，博士，美国实验动物医学院文凭，在密苏里大学接受了Lela Riley博士的博士培训，在那里她的研究重点是诊断分析的开发，毒力因素的识别，以及啮齿动物幽门螺杆菌感染的发病机制的表征。在SERCA，Beckwith博士将以她的研究背景为基础，并获得额外的培训，使她能够发展成为一名独立资助的研究调查员。Beckwith博士将在苏州丰富的知识环境中接受培训，在那里，国际知名的细菌发病机制研究领先者Stanley Falkow博士将指导她。贝克维斯博士是由兽医病理学家兼比较医学系主任琳达·科克博士共同赞助的，她将确保她拥有开展研究的资源和时间。临床和教学职责将仅足以维持她的临床技能。贝克维斯博士将进行一项深入的研究项目，在国家科学会议上展示她的研究成果，参加实验室会议，参加冷泉港实验室的专业课程，并参加与细菌发病机制相关的研讨会和期刊俱乐部。 Beckwith博士的研究将使用分子和遗传方法来识别和表征肝炎病毒、IBD、慢性活动性肝炎和小鼠肝细胞肿瘤的致病因素。肝包虫感染的小鼠是一种慢性细菌感染的动物模型，与人类肝胆和炎症性肠道疾病非常相似。贝克维斯博士将在肝型肝炎中创建一个转座子文库，并通过在小鼠身上进行负选择来识别毒力因子。新的毒力因子和宿主反应将使用传统和尖端技术来表征，包括利用微阵列进行全球基因表达分析。毒力因子的特征对于了解IBD和H引起的肝炎的发病机制是至关重要的。肝片。最近的证据表明，幽门螺杆菌与人类慢性肝胆疾病之间存在联系。该提案的长期目标是开发预防和治疗人类慢性肝脏和肠道疾病的新策略。